In vitro comparison of Enterococcus Faecalis survival in dentinal tubules following root canal therapy with AH plus, endoseal MTA, and cold ceramic sealers.

Autor: Mokhtari F; Department of Endodontics, Dental School, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.; School of Dentistry, Shahid Sadoughi University of Medical Sciences, Imam Reza Blvd., Imam Reza Sq, Yazd, Iran., Modaresi J; Department of Endodontics, Dental School, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.; School of Dentistry, Shahid Sadoughi University of Medical Sciences, Imam Reza Blvd., Imam Reza Sq, Yazd, Iran., Salmasi AH; Department of Endodontics, Dental School, AJA University of Medical Sciences, Tehran, Iran. endohasanzade@gmail.com.; Endodontics Department, AJA University of Medical Sciences, Dr. Fatemi St. West, Colonel Etemadzadeh St, Tehran, Tehran Province, Iran. endohasanzade@gmail.com., Khamisi N; School of Dentistry, Islamic Azad University (Khorasgan Branch), Isfahan, Iran.; Faculty of Dentistry, Islamic Azad University-Isfahan (Khorasgan) Branch, University boulevard, Arghavanie St., East Jey Sharghi St, P.O. Box 81595-158, Isfahan, 81551-39998, Iran., Zandi H; Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.; School of Medicine, Central Pardis of University, Shohadaye Gomnam Blvd, Safayee, Yazd, Iran., Lesani K; Department of Oral and Maxillofacial Radiology, School of Dentistry, Iran University of Medical Sciences, Azadi St., Jamalzadeh St., Jamshid Alley, Tehran, 1313883851, Iran.
Jazyk: angličtina
Zdroj: BMC oral health [BMC Oral Health] 2024 Dec 18; Vol. 24 (1), pp. 1488. Date of Electronic Publication: 2024 Dec 18.
DOI: 10.1186/s12903-024-05192-8
Abstrakt: Background: Enterococcus faecalis (E. faecalis) is the most common microorganism responsible for post-endodontic infections, which can penetrate deep into dentinal tubules. This study aimed to compare the survival of E. faecalis in dentinal tubules following root canal therapy with AH Plus (AHP), Endoseal MTA (ESM), and cold ceramic (CC) sealers.
Methods: In this in vitro experimental study, 80 single-canal human teeth were decoronated at the cementoenamel junction. The root canals were cleaned and shaped, and auto-clave sterilized after smear layer removal. The roots were then inoculated with E. faecalis, and incubated for 3 weeks. The teeth were randomly assigned to four groups (n = 20) for root canal obturation with AHP, ESM, and CC sealers and 2% gutta-percha with the cold lateral compaction technique. The fourth group served as the control group. After 30 days, dentin chips were collected from the canals, and cultured on blood agar. The colony count was reported and analyzed by the Kruskal-Wallis test (alpha = 0.05).
Results: The mean colony count was 23.73 ± 13.84 in the AHP, 34.78 ± 18.75 in the ESM, and 28.47 ± 13.73 in the CC group after 30 days. The difference in this regard was not significant among the three experimental groups (P = 0.102).
Conclusions: Within the limitations of this in vitro study, the results indicated comparable antibacterial activity of AHP, ESM, and CC sealers against E. faecalis in infected dentinal tubules after 30 days.
Competing Interests: Declarations. Ethics approval and consent to participate: The need for informed consent was waived by the ethics committee of Yazd Shahid Sadoughi University of Medical Sciences due to the use of anonymized extracted teeth. This study did not involve any human subjects directly and no identifiable patient information was used.”. The study protocol was approved by the ethics committee of Yazd Shahid Sadoughi University of Medical Sciences (IR.SSU.DENTISTRY.REC.1401.073). All methods were carried out in accordance with relevant guidelines and regulations. Consent for publication: Identified images or other personal or clinical details of participants are not presented in this manuscript. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s).)
Databáze: MEDLINE
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