Antimicrobial resistance and virulence factors analysis of a multidrug-resistant Acinetobacter baumannii isolated from chickens using whole-genome sequencing.
Autor: | Yu L; College of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China. yulumin@lyu.edu.cn., Zhao Y; College of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China., Zhang S; College of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China., Ni W; College of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China., Zhang L; College of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China., Xue C; College of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China., Wang P; College of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China., Zhang X; College of Agriculture and Forestry, Linyi University, Linyi, Shandong, 276005, China. zhangxinglin@lyu.edu.cn. |
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Jazyk: | angličtina |
Zdroj: | BMC microbiology [BMC Microbiol] 2024 Dec 19; Vol. 24 (1), pp. 526. Date of Electronic Publication: 2024 Dec 19. |
DOI: | 10.1186/s12866-024-03694-7 |
Abstrakt: | Multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii) is currently recognized not only as a significant nosocomial pathogen but also is an emerging bacterial infection in food-producing animals, posing a critical threat to global health. However, this is a hindrance to detailed bioinformatic studies of MDR A. baumannii of chicken origin due to the lack of its complete genome sequence. Here, we report whole-genome sequencing analysis of MDR A. baumannii Y03 isolated from chickens. The Y03 genome consists of 1 circular chromosome and 4 circular plasmids, The Y03 chromosome harbors 41 antimicrobial resistance genes conferring resistance to major classes of antibiotics, including β-lactams, phenicols, macrolides, lincosamides, aminoglycosides, and nitrofurans, as well as 135 virulence factors involved in effector delivery system, immune modulation, adherence, stress survival, biofilm, exotoxin, and nutritional/metabolic factor. The in vivo infection experiments certificated that Y03 was virulent to chickens. Meanwhile, we used PCR amplification method to detect 10 antimicrobial resistance genes including abeM, adeB, adeH, adeK, bla Competing Interests: Declarations. Ethics approval and consent to participate: This study was carried out in compliance with the ARRIVE guidelines. Animal experiments were conducted under animal protocols approved by the Institutional Animal Care and Use Committee (IACUC) of Linyi University (Approval No. LYU20240206). All animal work was carried out following accordance within the guidelines of the Laboratory Animal Research Center of Linyi University. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. (© 2024. The Author(s).) |
Databáze: | MEDLINE |
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