Innovative transgenic zebrafish biosensor for heavy metal detection.

Autor: Herath HMLPB; Centre for Advanced Materials and Devices (CAMD), Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka; School of Life and Environmental Sciences, Deakin University, Pigdons Road, Geelong, VIC, 3216, Australia; Department of Life Sciences, Faculty of Science, NSBM Green University, Homagama, Sri Lanka., de Silva RM; Centre for Advanced Materials and Devices (CAMD), Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka; Department of Life Sciences, Faculty of Science, NSBM Green University, Homagama, Sri Lanka., Dassanayake RS; Centre for Advanced Materials and Devices (CAMD), Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka., Gunawardene YINS; Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka., Jayasingha JRP; Centre for Advanced Materials and Devices (CAMD), Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka., Schultz A; School of Life and Environmental Sciences, Deakin University, Pigdons Road, Geelong, VIC, 3216, Australia., Liongue C; School of Medicine, Deakin University, Pigdons Road, Geelong, VIC, 3216, Australia., Ward AC; School of Medicine, Deakin University, Pigdons Road, Geelong, VIC, 3216, Australia., Afonso LOB; School of Life and Environmental Sciences, Deakin University, Pigdons Road, Geelong, VIC, 3216, Australia., de Silva KMN; Centre for Advanced Materials and Devices (CAMD), Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka. Electronic address: kmnd@chem.cmb.ac.lk.
Jazyk: angličtina
Zdroj: Environmental pollution (Barking, Essex : 1987) [Environ Pollut] 2024 Dec 16; Vol. 366, pp. 125547. Date of Electronic Publication: 2024 Dec 16.
DOI: 10.1016/j.envpol.2024.125547
Abstrakt: Heavy metal contamination is an urgent environmental issue that poses a significant threat to human health and the ecosystem. To mitigate the adverse impacts of heavy metal pollution, the aim of this research was to develop genetically engineered zebrafish as biosensors, which offer a promising alternative for detecting heavy metal exposure, specifically Cd 2 ⁺ and Zn 2 ⁺. A novel heavy metal-sensitive gene construct metallothionine 2 promoter with DsRed reporter gene (mt2-DsRed2) was synthesized and integrated into zebrafish embryos using a Tol2 transposon transposase system with the transgenic zebrafish line subjected to biosensing applications for Cd 2+ and Zn 2+ . The biosensor showed specific responses with linear correlation heavy metal concentration and DsRed fluorescence signal for Cd 2+ and Zn 2+ with (p < 0.01) a minimum detection limit of 4 ppb for each metal ion but not for the non-specific metal ion Ni 2+ , which makes it suitable for laboratory-based heavy metal assessment assays in the low heavy metal concentration ranges of 0-10 ppb. Additionally, the study investigated the toxicity of heavy metals on zebrafish early developmental stages applying a modified version of the OECD Fish Embryo Toxicity (FET-236) test. Accordingly, Cd 2+ , Zn 2+ , and Ni 2+ exhibited no significant toxicity effects on zebrafish embryos within the low dose range of 2-10 ppb confirming the biocompatibility of the transgenic zebrafish biosensor for heavy metal sensing applications. Thus, the developed transgenic zebrafish line can accurately sense heavy metals Cd 2+ and Zn 2+ within the low dose range, making it a promising alternative laboratory assay for environmental monitoring and risk assessment.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Prof. Rohini M. de Silva reports financial support, administrative support, and equipment, drugs, or supplies were provided by National Research Council of Sri Lanka. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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