Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches.

Autor: Acott TS; Department of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States., Fautsch MP; Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States., Mao W; Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States.; Departments of Biochemistry and Molecular Biology and Pharmacology and Toxicology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States., Ethier CR; Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States., Huang AS; Hamilton Glaucoma Center, The Viterbi Family Department of Ophthalmology, Shiley Eye Institute, University of California, San Diego, California, United States., Kelley MJ; Department of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States., Aga M; Department of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States., Bhattacharya SK; Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami, Florida, United States., Borras T; University of North Carolina, Chapel Hill, North Carolina, United States., Bovenkamp D; BrightFocus Foundation, Clarksburg, Maryland, United States., Chowdhury UR; Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States., Clark AF; North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, Texas, United States., Dibas MI; Glaucoma Research, Ophthalmology Discovery, AbbVie, Irvine, California, United States., Du Y; Morsani College of Medicine, University of South Florida, Tampa, Florida, United States., Elliott MH; Department of Ophthalmology and Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States., Faralli JA; Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, United States., Gong H; Department of Ophthalmology, Boston University School of Medicine, Boston, Massachusetts, United States., Herberg S; Department of Ophthalmology & Visual Sciences, SUNY Upstate Medical University, Syracuse, New York, United States., Johnstone MA; Department of Ophthalmology, University of Washington, Seattle, Washington, United States., Kaufman PL; Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States., Keller KE; Department of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States., Kelly RA; Department of Ophthalmology, Duke University, Durham, North Carolina, United States.; Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States., Krizaj D; Department of Ophthalmology and Visual Sciences, University of Utah, Salt Lake City, Utah, United States., Kuehn MH; Department Ophthalmology and Visual Sciences, Iowa City VA Center for Prevention and Treatment of Visual Loss, University of Iowa, Iowa City, Iowa, United States., Li HL; Department of Ophthalmology, Boston University School of Medicine, Boston, Massachusetts, United States., Lieberman R; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, United States., Lin SC; Glaucoma Center of San Francisco, San Francisco, California, United States., Liu Y; Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, Georgia, United States., McDonnell FS; John Moran Eye Center, University of Utah, Salt Lake City, Utah, United States., McDowell CM; Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States., McLellan GJ; Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, United States.; Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States., Mzyk P; Department of Ophthalmology, Duke University, Durham, North Carolina, United States.; Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States., Nair KS; Deptartment of Ophthalmology, University of California, San Francisco, San Francisco CA, United States., Overby DR; Department of Bioengineering, Imperial College of London, London, England., Peters DM; Department of Pathology and Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, United States., Raghunathan V; Department of Ophthalmology, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, United States., Rao PV; Department of Ophthalmology, Duke University, Durham, North Carolina, United States.; Department of Pharmacology and Cancer Biology, Duke University School of Medicine, Durham, North Carolina, United States., Roddy GW; Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota, United States., Sharif NA; Global Alliances and Collaborations, Ophthalmology Innovation Center, Santen Inc., Emeryville, California, United States., Shim MS; Department of Ophthalmology, Duke University, Durham, North Carolina, United States.; Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States., Sun Y; Department of Ophthalmology, Stanford University School of Medicine, Palo Alto, California, United States., Thomson BR; Department of Ophthalmology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, United States., Toris CB; Department of Ophthalmology and Visual Sciences, Ohio State University Wexner Medical Center, Columbus, Ohio, United States., Willoughby CE; Genomic Medicine, Biomedical Sciences Research Institute, Ulster University, Coleraine, Northern Ireland, United Kingdom., Zhang HF; Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, United States., Freddo TF; MCP Health Sciences University, Westport, Massachusetts, United States., Fuchshofer R; Institute for Human Anatomy and Embryology, University of Regensburg, Regensburg, Germany., Hill KR; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, United States., Karimi A; Department of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States., Kizhatil K; Department of Ophthalmology and Visual Science, Ohio State University, Columbus, Ohio, United States., Kopcyznski CC; Aerie Pharmaceuticals, Inc., Durham, North Carolina, United States., Liton P; Department of Ophthalmology, Duke University, Durham, North Carolina, United States., Patel G; North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, Texas, United States.; Department of Ophthalmology and Genetics Medicine Research, Regeneron Pharmaceuticals, Inc., Tarrytown, New York, United States., Peng M; Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States.; Departments of Biochemistry and Molecular Biology and Pharmacology and Toxicology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States., Pattabiraman PP; Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States., Prasanna G; Department of Ophthalmology, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, United States., Reina-Torres E; Department of Bioengineering, Imperial College of London, London, England., Samples EG; Western Glaucoma Foundation, Olympia, Washington, United States., Samples JR; Washington State College of Medicine, Spokane, Washington, United States., Steel CL; Qlaris Bio, Inc., Wellesley, Massachusetts, United States., Strohmaier CA; Department of Ophthalmology and Optometry, Kepler University Hospital, Johannes Kepler University, Linz, Austria., Subramanian P; BrightFocus Foundation, Clarksburg, Maryland, United States., Sugali CK; Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States.; Departments of Biochemistry and Molecular Biology and Pharmacology and Toxicology, Eugene and Marilyn Glick Eye Institute, Indiana University School of Medicine, Indianapolis, Indiana, United States., van Batenburg-Sherwood J; Department of Bioengineering, Imperial College of London, London, England., Wong C; Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States., Youngblood H; School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, United States., Zode GS; Department of Ophthalmology, University of California at Irvine, Irvine, California, United States., White E; Department of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, Oregon, United States., Stamer WD; Department of Ophthalmology, Duke University, Durham, North Carolina, United States.; Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States.
Jazyk: angličtina
Zdroj: Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2024 Dec 02; Vol. 65 (14), pp. 32.
DOI: 10.1167/iovs.65.14.32
Abstrakt: Intraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.
Databáze: MEDLINE