Development of a sensitive disease-screening model using comprehensive circulating microRNA profiles in dogs: A pilot study.
Autor: | Omura K; Scientific activity support team, ARKRAY Marketing Inc., Yousuien-nai, 59 Gansuin-cho, Kamigyo-ku, Kyoto 602-0008, Japan., Ide K; Tokyo University of Agriculture and Technology, Department of Veterinary Medicine, Laboratory of Veterinary Internal Medicine, 3-5-8 Saiwaicho, Fuchu City, Tokyo, Japan., Takahashi M; Joint Faculty of Veterinary Medicine, Kagoshima University Veterinary Teaching Hospital, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan., Furusawa Y; Joint Faculty of Veterinary Medicine, Kagoshima University Veterinary Teaching Hospital, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan., Kobayashi M; Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo, 180-8602, Japan., Miyagawa Y; Laboratory of Veterinary Internal Medicine II, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo 180-8602, Japan., Fujiwara-Igarashi A; Laboratory of Veterinary Radiology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino-shi, Tokyo 180-8602, Japan., Teshima T; Laboratory of Veterinary Internal Medicine, School of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan., Kubo Y; Veterinary Medical Teaching Hospital, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino-shi, Tokyo 180-8602, Japan., Yasuda A; Veterinary Medical Teaching Hospital, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino-shi, Tokyo 180-8602, Japan., Yoshida K; Veterinary Medical Teaching Hospital, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino-shi, Tokyo 180-8602, Japan., Hayakawa N; Veterinary Medical Teaching Hospital, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino-shi, Tokyo 180-8602, Japan., Kobayashi M; Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo, 180-8602, Japan., Momoi Y; Department of Veterinary Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan. |
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Jazyk: | angličtina |
Zdroj: | Veterinary and animal science [Vet Anim Sci] 2024 Nov 26; Vol. 27, pp. 100414. Date of Electronic Publication: 2024 Nov 26 (Print Publication: 2025). |
DOI: | 10.1016/j.vas.2024.100414 |
Abstrakt: | In the veterinary field, the utility of disease-identification models that use comprehensive circulating microRNA (miRNA) profiles produced through measurements based on next-generation sequencing (NGS) remains unproven. To integrate NGS technology with automated machine learning (autoML) to create a comprehensive circulating miRNA profile and to assess the clinical utility of a disease-screening model derived from this profile. The study involved dogs diagnosed with or being treated for various diseases, including tumors, across multiple veterinary clinics ( n = 254), and healthy dogs without apparent diseases ( n = 91). miRNA was extracted from EDTA-treated plasma, and a comprehensive analysis was conducted of one million reads per sample using NGS. Then autoML technology was applied to develop a diagnostic model based on miRNA. Among these models, the one with the highest performance was chosen for evaluation. The diagnostic model, based on the comprehensive circulating miRNA profile developed in this study, achieved an AUC score of 0.89, with a sensitivity of 85 % and a specificity of 88 % for the disease samples. The miRNA-based diagnostic model demonstrated high sensitivity for disease groups and has the potential to be an effective screening test. This study indicates that a comprehensive miRNA profile in dog plasma could serve as a highly sensitive blood biomarker. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Kohei Omura reports a relationship with Arkray Marketing Inc that includes: employment. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors. Published by Elsevier Ltd.) |
Databáze: | MEDLINE |
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