Oxidative Stress Monitoring Platform: A Longitudinal In vitro Multinuclear ( 1 H/ 19 F) MR Spectroscopic Study.
| Autor: | Mandal PK; Neuroimaging and Neurospectroscopy (NINS) Laboratory, National Brain Research Centre, Gurgaon, India. mandalpk@pitt.edu.; Department of Neurosurgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. mandalpk@pitt.edu.; Florey Institute of Neuroscience and Mental Health, Melbourne School of Medicine Campus, Melbourne, VIC, Australia. mandalpk@pitt.edu., Arora Y; Neuroimaging and Neurospectroscopy (NINS) Laboratory, National Brain Research Centre, Gurgaon, India., Samkaria A; Neuroimaging and Neurospectroscopy (NINS) Laboratory, National Brain Research Centre, Gurgaon, India.; Department of Forensic Science, Chandigarh University, Mohali, Punjab, India., Maroon JC; Department of Neurosurgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA., Fodale V; Department of Human Pathology of the Adult and Evolutive Age 'Gaetano Barresi', Section of Anesthesiology, University of Messina, Messina, Italy.; Departmental Operative Unit of Neuroanesthesia, University Hospital 'G.Martino' of Messina, Messina, Italy., Mehta Y; Department of Anesthesiology, Medanta Medicity, Gurgaon, Haryana, India., Chang YF; Department of Neurosurgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Cell biochemistry and biophysics [Cell Biochem Biophys] 2024 Dec 18. Date of Electronic Publication: 2024 Dec 18. |
| DOI: | 10.1007/s12013-024-01640-y |
| Abstrakt: | Glutathione (GSH) is a master antioxidant that counters oxidative stress. Clinical studies have confirmed significant depletion of GSH in the hippocampus and the substantia nigra as an early diagnostic biomarker for Alzheimer's disease (AD) and Parkinson disease (PD), respectively. External agents like anesthetics (inhaled and intravenous) have a different impact on GSH. There is significant depletion of the serum GSH peroxidase level after surgery with isoflurane anesthesia that is not found in patients administered intravenous propofol. The objective of this study is to evaluate the GSH level associated with isoflurane in vitro phantom model using non-invasive magnetic resonance (MR) spectroscopy and to detect residual isoflurane in a solution. MRS data was generated utilizing a 3T MR scanner (Prisma, Siemens) equipped with a 64-channel 1 H head coil and dual tune ( 19 F/ 1 H) head coil. The GSH data acquisition was performed using the MEGA-PRESS pulse sequence using experimental parameters: ON = 4.40 ppm, OFF = 5.00 ppm, TE = 120 ms, TR = 2500 ms, voxel size = 25 × 25 × 25 mm and average = 32. Isoflurane was detected using 19 F MRS studies using 19 F/ 1 H head coil. GSH data was processed using KALPANA package and 19 F data was processed using Siemens package. The GSH peak area (without isoflurane) in a phosphate-buffered solution (PBS) solution (control) showed a slow decline over time due to natural oxidation of GSH to dimeric glutathione (GSSG). On the contrary, the GSH peak area in similar model is reduced significantly (p = 0.016) due to isoflurane induced oxidation of GSH to GSSG compared to control. We also report a concise general method for data generation and processing of 1 H MRS data for GSH as well as 19 F monitoring platform using 19 F MR spectroscopy. This is the first report wherein both 1 H and 19 F spectroscopy are applied to generate MRS data along with a unique data processing method. This method is highly sensitive and specifically detects GSH without ambiguity as well as isoflurane due to the unique chemical shift patterns of CF Competing Interests: Compliance with Ethical Standards. Conflict of interest: The authors declare no competing interests. (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|
Full text from SpringerLink