Development of a natural rubber latex-based biodevice with mesenchymal stem cells as a potential treatment for skeletal muscle regeneration in gestational diabetes-induced myopathy.

Autor: Floriano JF; São Paulo State University (UNESP), Botucatu Medical School (FMB), Botucatu, São Paulo 18.618-687, Brazil; National Heart and Lung Institute, Imperial College London, London, UK; São Paulo State University (UNESP), Bioengineering & Biomaterials Group, School of Pharmaceutical Sciences (FCF), Araraquara, São Paulo 14800-903, Brazil. Electronic address: juliana.floriano@unesp.br., Barbosa AMP; São Paulo State University (UNESP), School of Philosophy and Sciences, Marília, São Paulo 17.525-900, Brazil., Emanueli C; National Heart and Lung Institute, Imperial College London, London, UK., de Lima PR; São Paulo State University (UNESP), Botucatu Medical School (FMB), Botucatu, São Paulo 18.618-687, Brazil., de Oliveira RG; São Paulo State University (UNESP), Botucatu Medical School (FMB), Botucatu, São Paulo 18.618-687, Brazil., De Carvalho CNF; São Paulo State University (UNESP), Botucatu Medical School (FMB), Botucatu, São Paulo 18.618-687, Brazil., Floriano EAF; Federal Institute of São Paulo (IFSP), Avaré, São Paulo 18707-150, Brazil., Zambuzzi WF; São Paulo State University (UNESP), Bioscienses Institute, Bioassays & Cell Dynam Lab, Dept Chem & Biochem, Botucatu, São Paulo 18.618-687, Brazil., Pinto TS; São Paulo State University (UNESP), Bioscienses Institute, Bioassays & Cell Dynam Lab, Dept Chem & Biochem, Botucatu, São Paulo 18.618-687, Brazil., Fernandes FH; São Paulo State University (UNESP), Botucatu Medical School (FMB), Botucatu, São Paulo 18.618-687, Brazil., Salvadori DMF; São Paulo State University (UNESP), Botucatu Medical School (FMB), Botucatu, São Paulo 18.618-687, Brazil., Magalhães PFC; São Paulo State University (UNESP), Botucatu Medical School (FMB), Botucatu, São Paulo 18.618-687, Brazil., Albano LGS; São Paulo State University (UNESP), Bauru School of Sciences (FC), Bauru, São Paulo 17033-360, Brazil., de Oliveira Graeff CF; São Paulo State University (UNESP), Bauru School of Sciences (FC), Bauru, São Paulo 17033-360, Brazil., Sant'Ana Pegorin Brasil G; São Paulo State University (UNESP), Bioengineering & Biomaterials Group, School of Pharmaceutical Sciences (FCF), Araraquara, São Paulo 14800-903, Brazil., Dos Santos LS; University of São Paulo (USP), Faculty of Philosophy, Sciences and Languages of Ribeirão Preto, 3900 Bandeirantes Avenue, Ribeirão Preto, SP 14.040-901, Brazil., Burd BS; São Paulo State University (UNESP), Bioengineering & Biomaterials Group, School of Pharmaceutical Sciences (FCF), Araraquara, São Paulo 14800-903, Brazil., Cao W; California State University Northridge (CSUN), College of Health and Human Development, CA 91324, USA., Herculano RD; São Paulo State University (UNESP), Bioengineering & Biomaterials Group, School of Pharmaceutical Sciences (FCF), Araraquara, São Paulo 14800-903, Brazil; California State University Northridge (CSUN), College of Health and Human Development, CA 91324, USA. Electronic address: rondinelli.herculano@unesp.br., de Assis Golim M; São Paulo State University (UNESP), Botucatu Medical School (FMB), Botucatu, São Paulo 18.618-687, Brazil., Ferreira Junior RS; São Paulo State University (UNESP), Center for the Study of Venoms and Venomous Animals (CEVAP), Botucatu, São Paulo 18610-307, Brazil., Sobrevia L; São Paulo State University (UNESP), Botucatu Medical School (FMB), Botucatu, São Paulo 18.618-687, Brazil; Cellular and Molecular Physiology Laboratory (CMPL), Department of Obstetrics, Division of Obstetrics and Gynaecology, School of Medicine, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago 8330024, Chile; Department of Physiology, Faculty of Pharmacy, Universidad de Sevilla, Seville E-41012, Spain; University of Queensland Centre for Clinical Research (UQCCR), Faculty of Medicine and Biomedical Sciences, University of Queensland, Herston, 4029, Queensland, Australia; Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, 9713GZ Groningen, the Netherlands; Tecnologico de Monterrey, Eutra, The Institute for Obesity Research (IOR), School of Medicine and Health Sciences, Monterrey, Nuevo León, Mexico. Electronic address: lsobrevia@uc.cl., Rudge MVC; São Paulo State University (UNESP), Botucatu Medical School (FMB), Botucatu, São Paulo 18.618-687, Brazil. Electronic address: marilzarudge@gmail.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Dec 15; Vol. 289, pp. 138777. Date of Electronic Publication: 2024 Dec 15.
DOI: 10.1016/j.ijbiomac.2024.138777
Abstrakt: Women with gestational diabetes mellitus show a high risk of developing Gestational Diabetes Induced Myopathy (GDiM). GDiM is characterized by significant pelvic floor skeletal muscle atrophy and urinary incontinence. This study aimed to develop a natural rubber latex (NRL) based biodevice with mesenchymal/stromal stem cells (MSCs) for skeletal muscle regeneration for women with GDiM. NRL showed porosity, roughness, biocompatibility, and bioactivity. MSCs adhesion on the NRL scaffold surface was assessed by scanning electron microscopy (SEM), confocal microscopy, and zymography. The scaffold's physicochemical and biological properties were carried out by Fourier transform infrared spectroscopy (FTIR), swelling and degradation studies, hemolytic activity, and antioxidant activity (AA), using Electronic Paramagnetic Resonance (EPR). MSCs in culture expressed CD90, adhered to plastic, differentiated, and produced fibroblast colonies. A high rate of cell proliferation was seen in MSCs on the NRL scaffold. FTIR analysis confirmed protein structures and polyisoprene in the scaffold. Swelling and degradation showed low water uptake and weight loss. Furthermore, NRL presented a hemolytic rate of 2.90 ± 0.26 % for 24 h, and EPR revealed the scaffold's strong AA. The generated biodevice has potential for muscle regeneration and may be useful as a therapeutic option for skeletal muscle disorders in GDiM or urinary incontinence.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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