The dose-response effect of lecithin on carotenoid bioaccessibility and Caco-2 cell uptake.

Autor: Li Z; Department of Food Science and Technology, The Ohio State University, Columbus, OH 43210, United States., Zhong S; Human Nutrition Program, The Ohio State University, Columbus, OH 43210, United States., Meshram N; Human Nutrition Program, The Ohio State University, Columbus, OH 43210, United States., Kopec RE; Human Nutrition Program, The Ohio State University, Columbus, OH 43210, United States; Foods for Health Discovery Theme, The Ohio State University, Columbus, OH 43210, United States. Electronic address: kopec.4@osu.edu.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Dec 04; Vol. 468, pp. 142347. Date of Electronic Publication: 2024 Dec 04.
DOI: 10.1016/j.foodchem.2024.142347
Abstrakt: Previous results have been mixed as to whether the emulsifying agent lecithin increases carotenoid bioaccessibility and Caco-2 cellular uptake. The dose-response effect of lecithin (0-5 mg) on carotenoid bioaccessibility and Caco-2 cellular uptake was investigated in vitro using a mixture of β-carotene, lycopene, lutein, zeaxanthin and astaxanthin. Resulting micelles were incubated with Caco-2 cells for 4 h. Carotenoids in chyme, micelle, and cellular fractions were quantitated using HPLC-DAD and HPLC-MS/MS. Lecithin-micelle interactions were visualized using confocal microscopy. A lecithin dose of 1 mg improved carotenoid bioaccessibility ∼2× and led to increased Caco-2 cell uptake of the carotenes tested, but no change in xanthophylls tested, as compared to the control group (P < 0.05). Doses of lecithin ≥3 mg did not improve carotenoid bioaccessibility or Caco-2 cell uptake and produced oil droplet aggregation. These results suggest that limited doses of lecithin should be investigated in relation to maximizing carotenoid bioavailability in humans.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Rachel E. Kopec reports financial support and equipment, drugs, or supplies were provided by Nu Skin Enterprises. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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