| Autor: |
Tynior W; Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 19 Jordana St., 41-808 Zabrze, Poland., Kłósek M; Department of Microbiology and Immunology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 19 Jordana St., 41-808 Zabrze, Poland., Salatino S; Molecular Biology Laboratories, Science and Innovation Platforms, Natural History Museum, London SW7 5BD, UK., Cuber P; Molecular Biology Laboratories, Science and Innovation Platforms, Natural History Museum, London SW7 5BD, UK., Hudy D; Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 19 Jordana St., 41-808 Zabrze, Poland., Nałęcz D; Department of Otolaryngology and Maxillofacial Surgery, St. Vincent De Paul Hospital, 1 Wójta Radtkego St., 81-348 Gdynia, Poland., Chan YT; Molecular Biology Laboratories, Science and Innovation Platforms, Natural History Museum, London SW7 5BD, UK., Gustave C; Molecular Biology Laboratories, Science and Innovation Platforms, Natural History Museum, London SW7 5BD, UK., Strzelczyk JK; Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 19 Jordana St., 41-808 Zabrze, Poland. |
| Abstrakt: |
Molar incisor hypomineralization (MIH) is a qualitative developmental defect that affects the enamel tissue of permanent molars and can also occur in permanent incisors. Enamel affected by MIH has reduced hardness, increased porosity, and a higher organic content than unaffected enamel. These characteristics predispose the enamel to accumulation of bacteria and a higher prevalence of caries lesions. Through a groundbreaking metagenomic analysis of the buccal mucosal sample from a patient with MIH, we explored the intricacies of its microbiome compared to a healthy control using state-of-the-art nanopore long-read sequencing. Out of the 210 bacterial taxa identified in the MIH microbiome, we found Streptococcus and Haemophilus to be the most abundant genera. The bacteria with the highest read counts in the patient with MIH included Streptococcus mitis , Haemophilus parainfluenzae , Streptococcus pneumoniae , Rothia dentocariosa , and Gemella haemolysans . Our results revealed a striking contrast between healthy and MIH affected children, with a higher dominance and number of pathogenic species ( S. pneumoniae , H. influenzae , and N. meningitidis ) and reduced diversity in the MIH-affected patient. This distinct microbial profile not only sheds light on MIH-affected patients, but paves the way for future research, inspiring deeper understanding and larger scale studies. |
