| Autor: |
Dong X; School of Agriculture, Yunnan University, Kunming 650500, China., Gao J; School of Agriculture, Yunnan University, Kunming 650500, China., Jiang M; School of Agriculture, Yunnan University, Kunming 650500, China., Tao Y; School of Agriculture, Yunnan University, Kunming 650500, China., Chen X; School of Agriculture, Yunnan University, Kunming 650500, China., Yang X; School of Agriculture, Yunnan University, Kunming 650500, China., Wang L; School of Agriculture, Yunnan University, Kunming 650500, China., Jiang D; School of Agriculture, Yunnan University, Kunming 650500, China., Xiao Z; Dehong Tropical Agriculture Research Institute, Dehong 678600, China., Bai X; Dehong Tropical Agriculture Research Institute, Dehong 678600, China., He F; School of Agriculture, Yunnan University, Kunming 650500, China. |
| Abstrakt: |
WUSCHEL-related homeobox ( WOX ) genes play significant roles in plant development and stress responses. Difficulties in somatic embryogenesis are a significant constraint on the uniform seedling production and genetic modification of Coffea arabica , hindering efforts to improve coffee production in Yunnan, China. This study comprehensively analyzed WOX genes in three Coffea species. A total of 23 CaWOXs , 12 CcWOXs , and 10 CeWOXs were identified. Transcriptomic profile analysis indicated that about half of the CaWOX genes were actively expressed during somatic embryogenesis. The most represented CaWOXs were CaWOX2a , CaWOX2b , CaWOX8a , and CaWOX8b , which are suggested to promote the induction and development of the embryogenic callus, whereas CaWOX13a and CaWOX13b are suggested to negatively impact these processes. Co-expression analysis revealed that somatic embryogenesis-related CaWOXs were co-expressed with genes involved in embryo development, post-embryonic development, DNA repair, DNA metabolism, phenylpropanoid metabolism, secondary metabolite biosynthesis, and several epigenetic pathways. In addition, qRT-PCR showed that four WOX genes responded to cold stress. Overall, this study offers valuable insights into the functions of CaWOX genes during somatic embryogenesis and under cold stress. The results suggest that certain WOX genes play distinct regulatory roles during somatic embryogenesis, meriting further functional investigation. Moreover, the cold-responsive genes identified here are promising candidates for further molecular analysis to assess their potential to enhance cold tolerance. |
