| Autor: |
Georg B; Department of Clinical Biochemistry, Bispebjerg University Hospital, 2400 Copenhagen, Denmark., Jørgensen HL; Department of Clinical Biochemistry, Amager and Hvidovre Hospital, 2650 Copenhagen, Denmark.; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark., Hannibal J; Department of Clinical Biochemistry, Bispebjerg University Hospital, 2400 Copenhagen, Denmark.; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark. |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of molecular sciences [Int J Mol Sci] 2024 Dec 03; Vol. 25 (23). Date of Electronic Publication: 2024 Dec 03. |
| DOI: |
10.3390/ijms252313006 |
| Abstrakt: |
Many endocrine glands exhibit circadian rhythmicity, but the interplay between the central circadian clock in the suprachiasmatic nucleus (SCN), the peripheral endocrine clock, and hormones is sparsely understood. We therefore studied the cellular localizations of the clock protein PER1, parathyroid hormone (PTH) and calcitonin (CT) in the parathyroid and thyroid glands, respectively. Thyroid glands, including the parathyroids, were dissected at different time-points from rats housed in 12 h:12 h light-darkness cycles, and were double-immunostained for PER1 and PTH or CT. Sera were analyzed for PTH, CT, phosphate, and calcium. In both glands, PER1 expression peaked late at night, while limited staining was seen during the daytime. High-resolution microscopy revealed cytosolic PER1 at zeitgeber time (ZT)12, and nucleic staining at ZT24 in both PTH and CT cells. PTH peaked at Z12-ZT16, while neither CT staining nor serum CT oscillated during the daily cycle. Serum PTH was significantly higher at ZT12 than ZT24, but only phosphate was found to exhibit significant diurnal oscillation. The staining of the calcium-sensitive receptor (CSR) did not demonstrate circadian oscillation. In conclusion, PER1 expression peaked late at night/early in the morning in hormone-producing cells of both the thyroid and parathyroid glands. In the parathyroids, this was preceded by a PTH peak, while neither CT nor CRS were found to oscillate. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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