Genome-Wide Identification of the CAT Genes and Molecular Characterization of Their Transcriptional Responses to Various Nutrient Stresses in Allotetraploid Rapeseed.

Autor: Du XQ; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China., Sun SS; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China., Zhou T; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China., Zhang L; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China., Feng YN; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China., Zhang KL; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China., Hua YP; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2024 Nov 25; Vol. 25 (23). Date of Electronic Publication: 2024 Nov 25.
DOI: 10.3390/ijms252312658
Abstrakt: Brassica napus is an important oil crop in China and has a great demand for nitrogen nutrients. Cationic amino acid transporters (CAT) play a key role in amino acid absorption and transport in plants. However, the CATs family has not been reported in B. napus so far. In this study, genome-wide analysis identified 22 CAT members in the B. napus genome. Based on phylogenetic and synteny analysis, BnaCATs were classified into four groups (Group I-Group IV). The members in the same subgroups showed similar physiochemical characteristics and intron/exon and motif patterns. By evaluating cis -elements in the promoter regions, we identified some cis -elements related to hormones, stress and plant development. Darwin's evolutionary analysis indicated that BnaCATs might have experienced strong purifying selection pressure. The BnaCAT family may have undergone gene expansion; the chromosomal location of BnaCATs indicated that whole-genome replication or segmental replication may play a major driving role. Differential expression patterns of BnaCATs under nitrate limitation, phosphate shortage, potassium shortage, cadmium toxicity, ammonium excess and salt stress conditions indicated that they were responsive to different nutrient stresses. In summary, these findings provide a comprehensive survey of the BnaCAT family and lay a foundation for the further functional analysis of family members.
Databáze: MEDLINE