| Autor: |
Orynbekova S; School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Almaty 050012, Kazakhstan., Kukula-Koch W; Department of Pharmacognosy with Medicinal Plant Garden, Medical University of Lublin, 1 Chod'zki Street, 20-093 Lublin, Poland., Sakipova Z; School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Almaty 050012, Kazakhstan., Alsharif B; School of Pharmacy and Pharmaceutical Sciences, Panoz Institute, and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, D02 PN40 Dublin, Ireland.; Faculty of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi Arabia., Rafferty B; School of Pharmacy and Pharmaceutical Sciences, Panoz Institute, and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, D02 PN40 Dublin, Ireland., Nurgozhin T; School of General Medicine, Asfendiyarov Kazakh National Medical University, Almaty 050012, Kazakhstan., Allambergenova Z; School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Almaty 050012, Kazakhstan., Dreher P; Department of Public Health, Faculty of Medicine, Medical University of Lublin, 20-093 Lublin, Poland., Głowniak K; Department of Cosmetology, The University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland., Boylan F; School of Pharmacy and Pharmaceutical Sciences, Panoz Institute, and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, D02 PN40 Dublin, Ireland. |
| Abstrakt: |
Adonis tianschanica is a lesser-known plant species belonging to the genus Adonis that grows in Kazakhstan. The aim of this study was to characterize the composition of the ethanolic, water, and hydroethanolic extracts from the aerial parts of A. tianschanica by HPLC-ESI-QTOF-MS/MS to isolate the major compound isoquercitrin by HSCCC (High-Speed Counter-Current Chromatography) and to determine the cytotoxicity and anti-inflammatory potential of the extracts produced with this plant. Fingerprinting of the analyzed extracts showed the presence of a multitude of metabolites comprising polyphenols, organic acids, and coumarins, and only trace quantities of cardiac glycosides in the analyzed samples. Flavonoids were certainly the best-represented group, with kaempferol, quercetin, and their derivatives as the major components of the extracts. Key findings in this paper were that the ethanol: water (50:50 v / v ) extract of A. tianschanica and its major compound isoquercitrin were able to reduce the production of NO induced by LPS, in addition to demonstrating anti-inflammatory effects by reducing cytokines such as IL-6, TNF-α, and IL-1β. |
