| Autor: |
Ibadullayeva AK; Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan., Kasela M; Department of Pharmaceutical Microbiology, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland., Kozhanova KK; Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan., Kadyrbayeva GM; Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan., Widelski J; Department of Pharmacognosy with Medicinal Plants Garden, Lublin Medical University, 20-093 Lublin, Poland., Wojtanowski K; Independent Laboratory of Chemistry of Natural Products, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland., Józefczyk A; Department of Pharmacognosy with Medicinal Plants Garden, Lublin Medical University, 20-093 Lublin, Poland., Suśniak K; Department of Pharmaceutical Microbiology, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland., Okińczyc P; Department of Pharmacognosy and Herbal Medicines, Wrocław Medical University, 211a Borowska Street, 50-556 Wrocław, Poland., Tleubayeva MI; Department of Organization and Management and Economics of Pharmacy and Clinical Pharmacy, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan., Karaubayeva AA; Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan., Zhandabayeva MA; Department of Pharmaceutical Technology, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan., Mukhamedsadykova AZ; Department of Engineering Disciplines of Good Practices, School of Pharmacy, Kazakh National Medical University, 88 Tole Bi Street, Almaty 050012, Kazakhstan., Malm A; Department of Pharmaceutical Microbiology, Medical University of Lublin, 1 Chodzki Street, 20-093 Lublin, Poland. |
| Abstrakt: |
The genus Inula has been used in folk medicine for centuries; however, the data concerning Inula britannica L. are scarce. This study aimed at investigating the chemical composition of methanolic and ethanolic extracts from the aerial parts of I. britannica collected in Kazakhstan and evaluating their antimicrobial and antioxidant properties, with special attention being paid to polyphenols. The total content of polyphenols and flavonoids in the extracts was determined colorimetrically, while their qualitative and quantitative analyses were conducted using HPLC/ESI-QTOF-MS and RP-HPLC/DAD. Their antioxidant potential was determined using the FRAP and DPPH methods, whereas their antimicrobial activity was determined by the microdilution method towards a panel of reference microorganisms, including pathogens of the human gastrointestinal tract. Chemical analysis demonstrated that the methanolic extract had a higher content of polyphenols (58.02 vs. 43.44 mg GAE/g) and flavonoids (21.69 vs. 13.91 mg QUE/g) than the ethanolic extract. In both extracts, 15 compounds were identified, with the highest contents being those of cynarine (13.96 and 11.68 mg/g) and chlorogenic acid (9.22 and 5.09 mg/g). The DPPH assay showed a higher antioxidant activity of the methanolic extract (19.78 ± 0.12 mg GAE/g) in comparison to that of the ethanolic extract (15.56 ± 0.24 mg GAE/g). Similarly, the FRAP method showed that the methanolic extract exerted a much higher antioxidant activity (5.07 ± 0.18 mmol Fe 2+ /g) than the ethanolic extract (0.39 ± 0.01 mmol Fe 2+ /g). In contrast, both extracts showed similar antimicrobial properties, with the highest activity being that against Helicobacter pylori ATCC 43504 (MIC = 0.125-0.25 mg/mL). This paper presents novel data on I. britannica L., implying its significance as a source of valuable active compounds and being a prerequisite for further biological studies. |
