| Autor: |
Wang T; College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China., Tian Z; College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China., Yu M; College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China., Zhang S; General Station of Animal Husbandry of Shandong Province, Jinan 250100, China.; Protection of Animal Genetic Resources and Biological Breeding Engineering Research Center of Shandong Province, Jinan 250300, China., Zhang M; General Station of Animal Husbandry of Shandong Province, Jinan 250100, China.; Protection of Animal Genetic Resources and Biological Breeding Engineering Research Center of Shandong Province, Jinan 250300, China., Zhai X; General Station of Animal Husbandry of Shandong Province, Jinan 250100, China.; Protection of Animal Genetic Resources and Biological Breeding Engineering Research Center of Shandong Province, Jinan 250300, China., Shen W; College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China., Wang J; College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China. |
| Abstrakt: |
There is a consensus that indigenous pigs in China are more resistant than modern commercial pigs in terms of disease resistance. Generally, the immune response is an important part of anti-disease capability; however, the related mechanism in pigs is largely puzzling. Here, the public transcriptome data of peripheral blood mononuclear cells (PBMCs) from Dapulian (Chinese local breed) and Landrace (Commercial breed) pigs after stimulation with polyinosinic-polycytidylic acid (poly I:C, a conventional reagent used for simulation of the viral infection) were reanalyzed, and the immune response mechanism in different pig breeds was investigated from a transcriptomic perspective. Of note, through comparative analyses of Dapulian and Landrace pigs, the candidate genes involved in swine broad-spectrum resistance were identified, such as TIMD4 , RNF128 and VCAM1 . In addition, after differential gene expression, target gene identification and functional enrichment analyses, a potential regulatory network of miRNA genes associated with immune response was obtained in Dapulian pigs, including five miRNAs and 12 genes (such as ssc-miR-181a, ssc-miR-486, IL1R1 and NFKB2 ). This work provides new insights into the immune response regulation of antiviral responses in indigenous and modern commercial pigs. |
