| Autor: |
Cheng S; School of Breeding and Multiplication, Sanya Institute of Breeding and Multiplication, Hainan University, Sanya 572025, China.; Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China., Tao K; Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China., Lv X; Institute of Agricultural Quality Standards and Testing Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China., Lai Z; Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China., Lv Y; Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China., Wang Z; Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China., Chen Q; School of Breeding and Multiplication, Sanya Institute of Breeding and Multiplication, Hainan University, Sanya 572025, China.; Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China. |
| Abstrakt: |
Oxathiapiprolin, a novel fungicide targeting oxysterol-binding proteins (OSBP), has proven to be effective against various oomycete diseases, but its effects on pathogens remain underexplored. In this study, we demonstrated that oxathiapiprolin inhibits mycelium growth, sporangium production, zoospore release, and oospore production in Peronophythora litchii , with 50% effective concentrations ranging from 0.000153 to 0.011681 μg mL -1 . Additionally, in vivo tests confirmed its efficacy in controlling litchi downy blight. Our study demonstrated that oxathiapiprolin disrupts cellular ultrastructure and plasma membrane integrity, affects stress sensitivity, inhibits sterol transport, increases autophagy levels, reduces lipid droplet content, and induces lipid metabolism disorders. Gene expression analysis revealed that oxathiapiprolin affects the expression of PlORP1 (ORP1 gene of P. litchii ), with docking simulations further confirming its interaction with the PlORP1 (ORP1 protein of P. litchii ) protein. Ultimately, this cascade of events led to pathogen death. This paper reveals the antioomycete mechanism of oxathiapiprolin against P. litchii . |
