Exploring the purification, characterization, and industrial applications of Exopolysaccharide (EPS) from Bacillus amyloliquefaciens strain BDIFST240014.

Autor: Bhowmik B; Principal Scientific Officer (PSO), Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-i-Khuda Road, Dhaka, 1205, Bangladesh., Afrin S; Principal Scientific Officer (PSO), Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-i-Khuda Road, Dhaka, 1205, Bangladesh., Jui AH; Principal Scientific Officer (PSO), Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-i-Khuda Road, Dhaka, 1205, Bangladesh., Bhuiyan RH; BCSIR Dhaka Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-i-Khuda Road, Dhaka, 1205, Bangladesh., Rashid MM; Principal Scientific Officer (PSO), Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-i-Khuda Road, Dhaka, 1205, Bangladesh., Miah MAS; Principal Scientific Officer (PSO), Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-i-Khuda Road, Dhaka, 1205, Bangladesh., Bhuiyan MNI; Principal Scientific Officer (PSO), Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-i-Khuda Road, Dhaka, 1205, Bangladesh. nazrulctg@bcsir.gov.bd.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Dec 16; Vol. 52 (1), pp. 51. Date of Electronic Publication: 2024 Dec 16.
DOI: 10.1007/s11033-024-10173-9
Abstrakt: Background: Exopolysaccharides (EPS) derived from microbial sources hold great promise for various industrial applications due to their biodegradability and diverse biological activities.
Methods and Results: In this study, EPS was isolated and characterized from Bacillus amyloliquefaciens strain BDIFST240014, a Gram-positive bacterium known for its robust EPS production. Taxonomic classification was confirmed through 16 S rRNA gene sequencing. We optimized the growth conditions for EPS production, yielding a maximum of approximately 74.81 mg/L after 48 h of incubation at 37 °C. UV-vis and FTIR spectroscopy were used to characterize the EPS, revealing key functional groups indicative of its polysaccharide nature. The EPS contained total carbohydrate and protein contents of 841.234 µg/5 mg and 630.54 µg/5 mg, respectively, along with a potent water holding capacity (WHC) of 103.31% and oil holding capacity (OHC) of 106.78%, underscoring its potential as a multifunctional biopolymer. Antimicrobial assays demonstrated significant activity against both Gram positive and Gram negative pathogens, including S. aureus, B. subtilis, E. coli, and S. typhi. Antioxidant assays confirmed its potent DPPH radical scavenging activity (71.52%) and Fe³⁺ reducing capacity. Furthermore, emulsification studies showed its efficacy with various oils (coconut oil, soybean oil, sunflower oil, olive oil) and a hydrocarbon (n-hexane), with the highest emulsification indices (E1 and E24) of 60.78% and 60.39%, respectively, against coconut and sunflower oils. Notably, the EPS selectively promoted the growth of probiotic strains while not enhancing the growth of pathogenic bacteria, highlighting its prebiotic potential.
Conclusions: These findings suggest that EPS from B. amyloliquefaciens BDIFST240014 is a promising candidate for diverse industrial and biomedical applications.
Competing Interests: Declarations. Ethical approval: This study did not involve any human or animal-related experiments, and therefore, no ethical approval was required. Informed consent: This consent was obtained from all individual persons included in the study. Consent for publication: Informed consent for publication was obtained from all individual authors. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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