Electrochemical biosensor for detection of ampicillin in milk based on Au 5 Pt and DNA cycle dual-signal amplification strategy.

Autor: Mingyao L; School of Life Sciences and Health Engineering, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China., Ruiyi L; School of Life Sciences and Health Engineering, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China. ruiyili@jiangnan.edu.cn., Zaijun L; School of Life Sciences and Health Engineering, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China. zaijunli@jiangnan.edu.cn.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Dec 16; Vol. 192 (1), pp. 15. Date of Electronic Publication: 2024 Dec 16.
DOI: 10.1007/s00604-024-06817-z
Abstrakt: A biosensor is reported for electrochemical detection of ampicillin based on Au 5 Pt and DNA cycle dual-signal amplification strategy. Firstly, Au 5 Pt is prepared by reduction of chloroauric acid and chloroplatinic acid with serine-functionalized graphene quantum dot (SGQD). The resulting Au 5 Pt shows an excellent catalytic activity because of the synergy of Au and Pt. Then, Au 5 Pt is covalently combined with hairpin DNA and thionine molecule to form a redox probe. The probe was used for construction of the ampicillin biosensor coupling with DNA cycling. In the presence of ampicillin, the DNA cycle was triggered and leads to many redox probes being carried to the biosensor. This produces a significant signal amplification by oxidation and reduction of thionine molecules in these probes. The combination of Au 5 Pt catalysis with DNA cycle achieve ultrahigh sensitivity, selectivity, and stability for the electrochemical detection of ampicillin. Differential pulse voltammetry current linearly increases with the increase of ampicillin concentration in the range 1 × 10 -18 -1 × 10 -12  M with a detection limit of 3.2 × 10 -19  M (S/N = 3). The proposed analytical method has been satisfactorily used for electrochemical detection of ampicillin in milk.
Competing Interests: Declarations. Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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