Diffractive hyperchromatic objective for chromatic confocal microscopy.

Autor:	Chen J; Wyant College of Optical Sciences, The University of Arizona, Tucson, Arizona 85721, USA., Li S; Wyant College of Optical Sciences, The University of Arizona, Tucson, Arizona 85721, USA., Kang W; Wyant College of Optical Sciences, The University of Arizona, Tucson, Arizona 85721, USA., Guan S; Wyant College of Optical Sciences, The University of Arizona, Tucson, Arizona 85721, USA., Hong Z; Wyant College of Optical Sciences, The University of Arizona, Tucson, Arizona 85721, USA., Liang R; Wyant College of Optical Sciences, The University of Arizona, Tucson, Arizona 85721, USA.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2024 Nov 14; Vol. 15 (12), pp. 6834-6844. Date of Electronic Publication: 2024 Nov 14 (Print Publication: 2024).
DOI:	10.1364/BOE.543322
Abstrakt:	The limited focal shift of conventional achromatic objectives constrains the maximum imaging depth of chromatic confocal microscopes. To address this, we designed a hyperchromatic confocal microscope using diffractive optical elements, which was fabricated by single-point diamond turning (SPDT). This design takes advantage of the small Abbe number of diffractive optical elements to introduce a significant longitudinal chromatic shift. The resulting chromatic confocal microscope achieved a maximum imaging depth of 750 µm and a lateral resolution of 0.78 µm across a wavelength range of 600-810 nm. The system's imaging capabilities were demonstrated by capturing detailed images of biological samples, including cucumber seed cavities, pig kidney, and human forearm skin. These results confirmed the microscope's effectiveness in visualizing key cellular structures, underscoring its potential for high-resolution biological imaging. Competing Interests: The authors declare that they have no conflict of interest. (© 2024 Optica Publishing Group.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu