Detection of human brain cancers using genomic and immune cell characterization of cerebrospinal fluid through CSF-BAM.
| Autor: | Pearlman AH, Wang Y, Kalluri A, Parker M, Cohen JD, Dudley J, Rincon-Torroella J, Xia Y, Gensler R, Horwitz MA, Theodore J, Dobbyn L, Popoli M, Ptak J, Silliman N, Judge K, Groves M, Jackson CM, Jackson EM, Jallo GI, Lim M, Luciano M, Mukherjee D, Naidoo J, Rozati S, Sterling CH, Weingart J, Koschmann C, Mansoori A, Glantz M, Kamson D, Schreck KC, Pardo CA, Holdhoff M, Paul S, Kinzler KW, Papadopoulos N, Vogelstein B, Douville C, Bettegowda C |
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| Jazyk: | angličtina |
| Zdroj: | MedRxiv : the preprint server for health sciences [medRxiv] 2024 Dec 04. Date of Electronic Publication: 2024 Dec 04. |
| DOI: | 10.1101/2024.12.02.24318303 |
| Abstrakt: | Patients who have radiographically detectable lesions in their brain or other symptoms compatible with brain tumors pose challenges for diagnosis. The only definitive way to diagnose such patients is through brain biopsy, an obviously invasive and dangerous procedure. Here we present a new workflow termed "CSF-BAM" that simultaneously identifies B cell or T cell receptor rearrangements, A neuploidy, and M utations using PCR-mediated amplification of both strands of the DNA from CSF samples. We first describe the details of the molecular genetic assessments and then establish thresholds for positivity using training sets of libraries from patients with or without cancer. We then applied CSF-BAM to an independent set of 206 DNA samples from patients with common, aggressive cancer types as well as other forms of brain cancers. Among the 126 samples from patients with the most common aggressive cancer types (high grade gliomas, medulloblastomas, or metastatic cancers to the brain), the sensitivity of detection was >81%. None of 33 CSF-BAM assays (100% specificity, 90% to 100% credible interval) were positive in CSF samples from patients without brain cancers. The sensitivity of CSF-BAM was considerably higher than that achieved with cytology. CSF-BAM provides an integrated multi-analyte approach to identify neoplasia in the central nervous system, provides information about the immune environment in patients with or without cancer, and has the potential to inform the subsequent management of such patients. Statement of Significance: There is a paucity of technologies beyond surgical biopsy that can accurately diagnose central nervous system neoplasms. We developed a novel, sensitive and highly specific assay that can detect brain cancers by comprehensively identify somatic mutations, chromosomal copy number changes, and adaptive immunoreceptor repertoires from samples of cerebrospinal fluid. |
| Databáze: | MEDLINE |
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