Two-dimensional impedance studies of BSA buoyant density separated human erythrocytes.

Autor: Leif RC, Schwartz S, Rodriguez CM, Pell-Fernandez L, Groves M, Leif SB, Cayer M, Crews H
Jazyk: angličtina
Zdroj: Cytometry [Cytometry] 1985 Jan; Vol. 6 (1), pp. 13-21.
DOI: 10.1002/cyto.990060104
Abstrakt: Combined DC (Coulter Volume) and radio frequency impedance studies were performed on human erythrocytes which had been separated by buoyant density in linear, neutral, isotonic bovine serum albumin gradients. The individual buoyant density fractions showed no reproducible shift in volume with buoyant density but did show a shift with opacity, radio frequency impedance divided by dc impedance. This new electronic parameter of opacity can be related to cell age, since both it and cell age are directly related to buoyant density. This increase in opacity with buoyant density is correlated with a change in shape.
Databáze: MEDLINE