OsIAA7 enhances heat stress tolerance by inhibiting the activity of OsARF6 in rice.

Autor: Qiu R; State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China., Yao P; State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China., Yang J; State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China., Hou J; State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China., Xiao H; State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China., Wu Y; State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China., Tu D; State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China., Ma X; State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China., Zhao Y; State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China., Li L; State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China. Electronic address: ljli@whu.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Dec 16; Vol. 288, pp. 138746. Date of Electronic Publication: 2024 Dec 16.
DOI: 10.1016/j.ijbiomac.2024.138746
Abstrakt: Heat stress (HS) severely affects the growth and yield of rice, necessitating a clear understanding of the molecular mechanisms underlying HS tolerance. In this study, we report that the Aux/IAA family gene, OsIAA7, whose expression is induced by HS and positively regulates HS tolerance in rice (Oryza sativa L.). The osiaa7 mutant exhibits reduced HS tolerance, whereas overexpression of OsIAA7 enhances it. Our findings suggest that OsIAA7 contributes to HS tolerance by reducing hydrogen peroxide accumulation and cell death. Physiological analysis indicates that OsIAA7 influences the levels of malondialdehyde, catalase, and chlorophyll A concentration in plants under HS conditions. RNA-seq analysis suggests that OsIAA7 modulates the expression of heat-responsive genes, contributing to HS tolerance. Further, biochemical analyses demonstrate a physical interaction between OsIAA7 and OsARF6, with OsIAA7 inhibiting the activity of OsARF6. RT-qPCR results support the notion that the positive regulatory factor OsIAA7 and the negative regulatory factor OsARF6 control HS tolerance by regulating the transcript levels of OsTT1 and OsTT3.1. Together, our results reveal the role of OsIAA7 in controlling HS tolerance through the modulation of physiological processes and the inhibition of OsARF6 activity, suggesting that some Aux/IAA family genes play a role in heat tolerance in rice.
Competing Interests: Declaration of competing interest The authors declare no competing interests.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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