Etched CoFe Prussian blue analog nanozymes with superior peroxidase activity for colorimetric biosensing of hydrogen peroxide and dopamine.

Autor: Ramgopal NC; MLR Institute of Technology, Hyderabad, Telangana 500043, India., Mukherjee P; Japan Advanced Institute of Science and Technology, Japan., Kamilya T; School of Mechatronics Engineering, Korea University of Technology & Education, 1600, Chungjeol-ro, Byeongcheon-myeon, Dongnam-gu, Cheonan-si 31253, Republic of Korea., Basavegowda N; Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea., Mahanthappa M; Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai 602105, Tamil Nadu, India. Electronic address: muttu.may1@gmail.com., Aljarba NH; Department of Biology, College of Sciences, Princess Nourah bint Abdulrahman University, Riyadh 11564, Saudi Arabia. Electronic address: nhaljarba@pnu.edu.sa., Alqahtani RA; Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Alkahtani S; Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Park J; School of Mechatronics Engineering, Korea University of Technology & Education, 1600, Chungjeol-ro, Byeongcheon-myeon, Dongnam-gu, Cheonan-si 31253, Republic of Korea. Electronic address: jhpark98@koreatech.ac.kr., Vishwanath RS; Centre for Research in Functional Materials (CRFM), JAIN (Deemed-to-be University), Jain Global Campus, Bengaluru 562112, Karnataka, India. Electronic address: vishwanath.rs@jainuniversity.ac.in.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Dec 12; Vol. 288, pp. 138766. Date of Electronic Publication: 2024 Dec 12.
DOI: 10.1016/j.ijbiomac.2024.138766
Abstrakt: Nanozymes represent a compelling alternative to natural enzymes due to their exceptional stability and cost-efficiency in diagnostic and therapeutic applications. In this work, a nanozyme etched CoFe Prussian blue analog (etched PBA) was designed and fabricated by a simple approach involving first synthesizing CoFe PBA and then chemical etching. The fabricated etched PBA acts as a peroxidase mimic catalyst. Compared to the naturally occurring enzyme (horseradish peroxidase), the etched PBA exhibited improved peroxidase-like catalytic efficiency for oxidizing 3,3',5,5'-tetramethylbnmenzidine (TMB). Particularly, the exceptional peroxidase-like activity of the etched PBA is attributed to modifications in its electronic and structural properties, which improved affinity for hydrogen peroxide (H 2 O 2 ) and TMB. Hence, these findings highlight etched PBA as a promising peroxidase mimic, enabling a colorimetric assay for real-time H 2 O 2 and dopamine (DA) detection. H 2 O 2 was detected via PBA-catalyzed TMB oxidation (bluish-green color), while DA monitoring occurred through ox-TMB reduction. The detection thresholds for H 2 O 2 and DA were determined to be 0.083 μM and 0.05 μM, respectively, with broad linear ranges of 0.1 μM - 4.0 mM for H 2 O 2 and 0.1-240 μM for DA. In addition, the etched PBA was validated for real sample analysis, showing potential for enzyme catalysis, biosensing, and colorimetric analysis of pharmaceuticals and biomolecules.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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