Comparation of pheromone-binding proteins 1 and 2 of Spodoptera frugiperda in perceiving the three sex pheromone components Z9-14:Ac, Z7-12: Ac and Z11-16: Ac.

Autor: Hu L; Zhongkai University of Agriculture and Engineering, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, PR China., Zhang T; Zhongkai University of Agriculture and Engineering, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, PR China., Wu Q; State Key Laboratory of Vegetable Biobreeding, Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China., Liang K; Zhongkai University of Agriculture and Engineering, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, PR China., Yu G; Zhongkai University of Agriculture and Engineering, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, PR China., He M; Zhongkai University of Agriculture and Engineering, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, PR China., Chen D; Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Xingangxilu 105, Guangzhou 510260, China., Su X; Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences & Key Laboratory of Green Prevention and Control of Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs & Key Laboratory of High Technology for Plant Protection of Guangdong Province, Guangzhou 510640, China., Zhang Y; Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences & Key Laboratory of Green Prevention and Control of Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs & Key Laboratory of High Technology for Plant Protection of Guangdong Province, Guangzhou 510640, China., Zhang Z; Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences & Key Laboratory of Green Prevention and Control of Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs & Key Laboratory of High Technology for Plant Protection of Guangdong Province, Guangzhou 510640, China., Shen J; Zhongkai University of Agriculture and Engineering, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs, PR China. Electronic address: shenjianmei@zhku.edu.cn.
Jazyk: angličtina
Zdroj: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2024 Dec; Vol. 206, pp. 106183. Date of Electronic Publication: 2024 Oct 24.
DOI: 10.1016/j.pestbp.2024.106183
Abstrakt: Pheromone-binding proteins (PBPs) are mainly responsible for binding and transporting hydrophobic pheromone molecules across the aqueous sensilla lymph to the receptor proteins. The preference of each PBP is believed to be different for each pheromone component within a single species. Significantly higher expression level of PBP1 and PBP2 in the male antennae of Spodoptera frugiperda suggesting that SfruPBP1 and SfruPBP2 might play important roles in pheromone perception. However, the preference of these two PBP to the three main pheromone components Z9-14: Ac, Z7-12: Ac and Z11-16: Ac have not been determined. In this study, a fluorescence competitive binding assay revealed that the binding intensities of SfruPBP1 and SfruPBP2 to Z9-14: Ac or Z7-12: Ac was comparable. We then used the CRISPR/Cas9 system to individually or simultaneously knock out PBP1 and PBP2 in S.frugiperda. The result of courtship behavior indicated that SfruPBP1 and SfruPBP2 were indispensable and played equal roles in perceiving the pheromones Z9-14: Ac and Z7-12: Ac for orientation, wing vibration, and hair-pencil display. Compared with Z9-14:Ac and Z7-12: Ac, Z11-16: Ac showed higher or medium binding intensities with SfruPBP1 and SfruPBP2 but played a minor role in inducing the wing vibration behavior. The results of this study are valuable for elucidating the mechanisms involved in sex pheromone perception and may facilitate the development of PBP-targeted pest control techniques.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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