Phenotypic and genotypic characterization of Candida species from the oral cavity of healthy individuals in Lublin province, Poland.
| Autor: | Andrzejuk P; Innovation Research Centre, John Paul II University in Biała Podlaska, Biala Podlaska, Poland., Tokarska-Rodak M; Faculty of Health Sciences, John Paul II University in Biała Podlaska, Biala Podlaska, Poland., Dyrda A; Innovation Research Centre, John Paul II University in Biała Podlaska, Biala Podlaska, Poland., Zarębska M; Innovation Research Centre, John Paul II University in Biała Podlaska, Biala Podlaska, Poland. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of oral microbiology [J Oral Microbiol] 2024 Dec 09; Vol. 17 (1), pp. 2437335. Date of Electronic Publication: 2024 Dec 09 (Print Publication: 2025). |
| DOI: | 10.1080/20002297.2024.2437335 |
| Abstrakt: | Background: Candida spp., particularly C. albicans , are commonly isolated fungi in the oral cavity. However, their prevalence in healthy participants and their genotype-phenotype relation remains elusive. Aim: This study aimed to update the information on Candida species colonizing the oral cavity of healthy population, identify the most common species, and characterize the intraspecific diversity to determine the genotype-phenotype relationship. Methods: Oral swabs of healthy participants who declared an absence of oral infection were analyzed. Microbiological methods: chromogenic media, sugar assimilation tests, drug susceptibility, filamentation tests, temperature tolerance analysis, and assessment of biofilm formation ability. Genotyping methods: PCR amplification of the internal transcribed spacer (ITS) region with MspI restriction enzyme digestion and 25S rDNA region. Results: Of the 500 individuals tested, 130 harbored C. albicans in 77%, C. dubliniensis in 12%, Pichia kudriavzevii (previously C. krusei ) in 8%, and Nakaseomyces glabrata (previously C. glabrata ) in 3%. The microbiological tests yielded conflicting results. Analysis of the 25S rDNA transposable intron region contributed to the identification of individual Candida spp. and intraspecific identification of C. albicans genotypes. Genotype A accounted for 70% ( n = 100) of C. albicans isolates, whereas genotypes B, C, and D ( C. dubliniensis ) accounted for 17%, 9%, and 4% of the isolates, respectively. Conclusion: The results indicate a complex genotype-phenotype relationship in Candida spp. and recommends combining microbiological and molecular methods for the efficient typing of Candida spp. Competing Interests: No potential conflict of interest was reported by the author(s). (© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.) |
| Databáze: | MEDLINE |
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