Uterine Transplant Optimization From a Preclinical Donor Model With Controlled Cardiocirculatory Arrest.

Autor:	Loiseau E; CRT2I UMR 1064, Nantes Université, CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology, Nantes, France.; Department of Gynecology, Nantes University Hospital, Nantes, France., Mesnard B; CRT2I UMR 1064, Nantes Université, CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology, Nantes, France.; Department of Gynecology, Nantes University Hospital, Nantes, France., Bruneau S; CRT2I UMR 1064, Nantes Université, CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology, Nantes, France., De Sousa C; Department of Gynecology, Rennes University Hospital, Hôpital Sud, Rennes, France., Bernardet S; CRT2I UMR 1064, Nantes Université, CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology, Nantes, France., Hervouet J; CRT2I UMR 1064, Nantes Université, CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology, Nantes, France., Minault D; CRT2I UMR 1064, Nantes Université, CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology, Nantes, France., Levy S; CRT2I UMR 1064, Nantes Université, CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology, Nantes, France., Le Gal A; Department of Radiology, Nantes University Hospital, Nantes, France., Dion L; Department of Gynecology, Rennes University Hospital, Hôpital Sud, Rennes, France.; Irset - Inserm UMR_S 1085, Rennes, France., Blancho G; CRT2I UMR 1064, Nantes Université, CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology, Nantes, France., Lavoue V; Department of Gynecology, Rennes University Hospital, Hôpital Sud, Rennes, France.; Irset - Inserm UMR_S 1085, Rennes, France., Branchereau J; CRT2I UMR 1064, Nantes Université, CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology, Nantes, France.; Department of Urology and Transplantation Surgery, Nantes University Hospital, Nantes, France.
Jazyk:	angličtina
Zdroj:	Transplantation direct [Transplant Direct] 2024 Dec 10; Vol. 11 (1), pp. e1735. Date of Electronic Publication: 2024 Dec 10 (Print Publication: 2025).
DOI:	10.1097/TXD.0000000000001735
Abstrakt:	Background: Uterus transplantation from deceased donors offers a promising solution to the organ shortage, but optimal preservation methods are crucial for successful outcomes. Our primary objective is to conduct an initial assessment of the contribution of oxygenated hypothermic perfusion in uterine transplantation. Methods: We performed a preclinical study on a porcine model of controlled donation after circulatory death (60 min warm ischemia). Ten uterus grafts were preserved for 12 h using static cold storage or hypothermic machine perfusion (VitaSmart device, perfusion pressure at 15 mm Hg). Subsequently, they were reperfused using ex vivo normothermic machine perfusion (Liverassist, perfusion pressure at 30 mm Hg) with oxygenated autologous blood to assess early ischemia/reperfusion injury. Not only resistance index assessment and oxygenation evaluation but also immunochemistry and gene expression analysis were performed. Results: This study demonstrates the feasibility of using hypothermic machine perfusion for uterine graft preservation, showing improvements in reperfusion capacity (decrease of resistance indexes; P  < 0.0001) and tissue oxygenation (higher oxygen level) compared with static cold storage. Conclusions: These findings provide valuable insights for further research and refinement of uterine transplantation procedures. Competing Interests: E.M. received technical support from Bridge to Life and Organ assist societies. The other authors declare no conflicts of interest. (Copyright © 2024 The Author(s). Transplantation Direct. Published by Wolters Kluwer Health, Inc.)
Databáze:	MEDLINE
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