Click-ready iridium(iii) complexes as versatile bioimaging probes for bioorthogonal metabolic labeling.

Autor:	Rigolot V; Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle Lille France cedric.lion@univ-lille.fr., Simon C; Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle Lille France cedric.lion@univ-lille.fr., Bouchet A; Univ. Lille, CNRS, UMR 8516 - LASIRe - Laboratoire Avancé de Spectroscopie pour les Interactions la Réactivité et l'Environnement Lille France., Lancel L; Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle Lille France cedric.lion@univ-lille.fr., Di Battista V; DTU Sustain, Technical University of Denmark Building 115, Kgs. Lyngby Denmark., Karpov D; Univ. Grenoble Alpes, INSERM, UA 07 Synchrotron Radiation for Biomedicine (STROBE) Grenoble ID16A France.; Nano-imaging beamline, European Synchrotron Radiation Facility Grenoble France., Vauzeilles B; Chemical Biology Department, Univ. Paris-Saclay, CNRS, Institut de Chimie des Substances Naturelles UPR 2301 91198 Gif-sur-Yvette France., Spriet C; Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle Lille France cedric.lion@univ-lille.fr.; Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille US 41 - UAR 2014 - PLBS Lille France., Sliwa M; Univ. Lille, CNRS, UMR 8516 - LASIRe - Laboratoire Avancé de Spectroscopie pour les Interactions la Réactivité et l'Environnement Lille France.; LOB, CNRS, INSERM, Ecole Polytechnique, Institut Polytechnique de Paris, Palaiseau France., Bohic S; Univ. Grenoble Alpes, INSERM, UA 07 Synchrotron Radiation for Biomedicine (STROBE) Grenoble ID16A France.; Nano-imaging beamline, European Synchrotron Radiation Facility Grenoble France., Biot C; Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle Lille France cedric.lion@univ-lille.fr., Lion C; Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle Lille France cedric.lion@univ-lille.fr.
Jazyk:	angličtina
Zdroj:	RSC chemical biology [RSC Chem Biol] 2024 Dec 03. Date of Electronic Publication: 2024 Dec 03.
DOI:	10.1039/d4cb00255e
Abstrakt:	Herein, we report the synthesis, photophysical characterization and validation of iridium(iii)-polypyridine complexes functionalized for click chemistry and bioorthogonal chemistry, as well as their versatile applications as probes in bioimaging studies exploiting metabolic labeling. The designed dyes are conjugated to chemical reporters in a specific manner within cells by CuAAC ligation and display attractive photophysical properties in the UV-visible range. They are indeed highly photostable and emit in the far-red to near-IR region with long lifetimes and large Stokes shifts. We demonstrate that they can be efficiently used to monitor nascent intracellular sialylated glycoconjugates in bioorthogonal MOE studies with a varied panel of optical and non-optical techniques, namely conventional UV-vis laser scanning confocal microscopy (for routine purposes), UV-vis time-resolved luminescence imaging (for specificity and facilitated multiplexing with nano-environment sensitivity), synchrotron radiation based X-ray fluorescence nanoimaging (for high resolution, elemental mapping and quantification in situ ) and inductively coupled plasma mass spectrometry (for routine quantification on cell populations with high statistical confidence). The synthesized Ir(iii) complexes were utilized in single labeling experiments, as well as in dual click-labeling experiments utilizing two distinct monosaccharide reporters relevant to the same metabolic pathway. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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