Simultaneous removal of methane and high nitrite from the wastewater by Methylomonas sp. With soluble methane monooxygenase.

Autor: Sang Y; Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China., Hao Q; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, PR China., Zhang Y; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, PR China., Wang O; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, PR China., Zheng S; Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China., Liu F; Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, PR China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, 266237, PR China. Electronic address: fhliu@soil.gd.cn.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2024 Dec 10, pp. 131972. Date of Electronic Publication: 2024 Dec 10.
DOI: 10.1016/j.biortech.2024.131972
Abstrakt: Aerobic methanotrophs play a crucial role in controlling methane emission in wastewater treatment. However, the high nitrite produced during ammonium oxidation, nitrate assimilation, and denitrification hinders methane oxidation and nitrogen removal. In this study, Methylomonas sp. HYX-M1, possessing two methane monooxygenase and multiple nitrite reductase genes, demonstrated efficient methane oxidation, coupled with nitrite removal abilities up to 6 mM. HYX-M1 presented methane oxidation rate of 0.05 mmol/d and nitrite removal rate of 0.53 mM/d under low-oxygen conditions. Assimilation and denitrification mainly accounted for 94.6-96.06 % and 3.10-5.03 % of nitrite removal. Methane monooxygenase genes, pmoA and mmoX expressed in different nitrite. Meanwhile, the nirB and nirD of strain HYX-M1 upregulated by 2.7- and 8.5-fold in 6 mM, respectively. The sod and ahpC genes upregulated, contributing to the survival of strain HYX-M1 in high nitrite. These findings provide a new strategy for the application of aerobic methanotrophs in regulating methane emission of wastewater with high nitrite.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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