Prognostic implication of CD47 and CTLA-4 expressions in endometrial carcinoma.

Autor: Ahmed MM; Pathology Department, Faculty of Medicine, Zagazig University, Egypt., Hussein S; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Egypt., Abdalsalam MM; Obestetrics and Gynaecology Department, El-Ahrar Teaching Hospital, Egypt., Sameh H; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Egypt., Waley AB; Medical Oncology Department, Faculty of Medicine, Zagazig University, Egypt., Ebian HF; Clinical Pathology Department, Faculty of Medicine, Zagazig University, Egypt., Sakr MMH; Clinical Pathology Department, Faculty of Medicine, Zagazig University, Egypt., Attia RN; Obstetrics and Gynaecology Department, Faculty of Medicine, Zagazig University, Egypt., Sameh R; Pathology Department, Faculty of Medicine, Zagazig University, Egypt.
Jazyk: angličtina
Zdroj: Human immunology [Hum Immunol] 2024 Dec 11; Vol. 86 (1), pp. 111210. Date of Electronic Publication: 2024 Dec 11.
DOI: 10.1016/j.humimm.2024.111210
Abstrakt: Background: CD47 is an immune-regulatory protein that belongs to the immunoglobulin family. It inhibits the phagocytic ability of immune cells. So, it is related to an unfavorable outcome in leukemia and various solid tumors. One of the immune checkpoint molecules is cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) which interferes with anticancer immunity and has an important role in anti-cancer surveillance. This study aimed to investigate CD47 and CTLA-4 mRNA and protein expressions in relation to P53 mutation and different clinicopathological parameters in endometrial carcinoma (EC). We also investigated the relation between CD47 and CTLA-4 expressions in EC.
Subject & Method: This study included sixty-eight patients with EC. Tissue samples of the tumor with adequate safety margin were obtained. Part of the tissues was preserved in formalin for histopathological and immunohistochemical examination while the other part was kept frozen at -80 °C for molecular profile.
Results: CD47 and CTLA-4 gene expressions were upregulated in the tissues of EC in comparison with the adjacent control tissues. Significantly higher CD47 and CTLA-4 gene expressions were detected in the serous type, higher stage, muscle invasion ≥50 %, higher grade, LN metastasis, and distant metastasis. CD47 gene expression was a good marker of P53 mutation at a cut-off of 1.65. It showed a high sensitivity of 84 %, a high specificity of 75.3 %, an average PPV of 65.6 %, a high NPV of 88.9 %, and an accuracy of 77.9 % (P < 0.001). Similarly, CTLA-4 gene expression was a good marker of P53 mutation at a cut-off of 3.75. It showed a sensitivity of 88 %, a specificity of 74.3 %, a PPV of 66.7 %, an NPV of 91.4 %, and an accuracy of 79.4 % (P < 0.001).
Conclusion: CD47 and CTLA-4 expressions can be considered possible diagnostic and prognostic markers in EC. They were good markers of P53 mutation, and higher tumor grades and stages.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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