Field EPSPs of Dentate Gyrus Granule Cells Studied by Selective Optogenetic Activation of Hilar Mossy Cells in Hippocampal Slices.

Autor: Bernstein HL; Departments of Child & Adolescent Psychiatry, Neuroscience & Physiology, and Psychiatry, and the Neuroscience Institute, New York University Langone Health, New York, New York, USA.; Center for Dementia Research, The Nathan Kline Institute for Psychiatric Research, New York State Office of Mental Health, Orangeburg, New York, USA., Lu YL; Departments of Child & Adolescent Psychiatry, Neuroscience & Physiology, and Psychiatry, and the Neuroscience Institute, New York University Langone Health, New York, New York, USA.; Center for Dementia Research, The Nathan Kline Institute for Psychiatric Research, New York State Office of Mental Health, Orangeburg, New York, USA., Botterill JJ; Departments of Child & Adolescent Psychiatry, Neuroscience & Physiology, and Psychiatry, and the Neuroscience Institute, New York University Langone Health, New York, New York, USA.; Center for Dementia Research, The Nathan Kline Institute for Psychiatric Research, New York State Office of Mental Health, Orangeburg, New York, USA., Duffy ÁM; Departments of Child & Adolescent Psychiatry, Neuroscience & Physiology, and Psychiatry, and the Neuroscience Institute, New York University Langone Health, New York, New York, USA.; Center for Dementia Research, The Nathan Kline Institute for Psychiatric Research, New York State Office of Mental Health, Orangeburg, New York, USA., LaFrancois JJ; Departments of Child & Adolescent Psychiatry, Neuroscience & Physiology, and Psychiatry, and the Neuroscience Institute, New York University Langone Health, New York, New York, USA.; Center for Dementia Research, The Nathan Kline Institute for Psychiatric Research, New York State Office of Mental Health, Orangeburg, New York, USA., Scharfman HE; Departments of Child & Adolescent Psychiatry, Neuroscience & Physiology, and Psychiatry, and the Neuroscience Institute, New York University Langone Health, New York, New York, USA.; Center for Dementia Research, The Nathan Kline Institute for Psychiatric Research, New York State Office of Mental Health, Orangeburg, New York, USA.
Jazyk: angličtina
Zdroj: Hippocampus [Hippocampus] 2025 Jan; Vol. 35 (1), pp. e23652.
DOI: 10.1002/hipo.23652
Abstrakt: Glutamatergic dentate gyrus (DG) mossy cells (MCs) innervate the primary DG cell type, granule cells (GCs). Numerous MC synapses are on GC proximal dendrites in the inner molecular layer (IML). However, field recordings of the GC excitatory postsynaptic potential (fEPSPs) have not been used to study this pathway selectively. Here we describe methods to selectively activate MC axons in the IML using mice with Cre recombinase expressed in MCs. Slices were made after injecting adeno-associated virus (AAV) encoding channelrhodopsin (ChR2) in the DG. In these slices, we show that fEPSPs could be recorded reliably in the IML in response to optogenetic stimulation of MC axons. Furthermore, fEPSPs were widespread across the septotemporal axis. However, fEPSPs were relatively weak because they were small in amplitude and did not elicit a significant population spike in GCs. They also showed little paired pulse facilitation. We confirmed the extracellular findings with patch clamp recordings of GCs despite different recording chambers and other differences in methods. Together the results provide a simple method for studying MC activation of GCs and add to the evidence that this input is normally weak but widespread across the GC population.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
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