Evaluation of binding activities of a putative lipoprotein LIC_13355 of Leptospira spp.

Autor: Silva IRM; Laboratório de Desenvolvimento de Vacinas, Instituto Butantan, São Paulo, Brazil.; Programa de Pós-Graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Brazil., Takahashi MB; Laboratório de Desenvolvimento de Vacinas, Instituto Butantan, São Paulo, Brazil., Teixeira AF; Laboratório de Desenvolvimento de Vacinas, Instituto Butantan, São Paulo, Brazil., Nascimento ALTO; Laboratório de Desenvolvimento de Vacinas, Instituto Butantan, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: FEBS open bio [FEBS Open Bio] 2024 Dec 12. Date of Electronic Publication: 2024 Dec 12.
DOI: 10.1002/2211-5463.13942
Abstrakt: Pathogenic Leptospira is the etiological cause of the zoonotic life-threatening infection called leptospirosis. The disease is spread worldwide with higher risk in tropical regions. Although leptospirosis represents a burden to the health of humans and animals, the pathogenic mechanisms of Leptospira infection are yet to be clarified. Leptospirosis infection is multifactorial, involving functionally redundant proteins with the capability to invade, disseminate, and escape the host's immune response. In this work, we describe a putative lipoprotein encoded by the gene LIC_13355, genome annotated as hypothetical of unknown function. The coding sequence is conserved among pathogenic Leptospira spp. with high percentage of coverage and identity. The recombinant protein, rLIC_13355, was expressed in Escherichia coli host system in its insoluble form. The circular dichroism spectrum of the refolded protein showed it containing a mixture of secondary structures. rLIC_13355 interacts with extracellular matrix (ECM) component laminin and binds plasminogen (PLG), generating plasmin (PLA), thus possibly participating during the adhesion and dissemination processes. The rLIC_13355 has the ability to interact with Ea.hy926 and HMEC-1 endothelial cells either in monolayer or suspension. The binding of rLIC_13355 with monolayer cells is dose-dependent on protein concentration. Taken together, our data suggest that this is presumably an adhesion lipoprotein that may play diverse roles in host-Leptospira interactions by mediating the interaction with host components and with endothelial cell.
(© 2024 The Author(s). FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
Databáze: MEDLINE
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