Reaction-Based Fluorescence Assays for the Determination of Food Trimethylamine Oxide.

Autor: Peng D; School of Pharmaceutical Sciences, Artemisinin Research Center, Guangzhou University of Chinese Medicine, Guangzhou, China., Huang X; Chemical Engineering College, Nanjing University of Science & Technology, Nanjing, China., Wang Q; School of Pharmaceutical Sciences, Artemisinin Research Center, Guangzhou University of Chinese Medicine, Guangzhou, China., Huang L; School of Pharmaceutical Sciences, Artemisinin Research Center, Guangzhou University of Chinese Medicine, Guangzhou, China., Liu F; School of Pharmaceutical Sciences, Artemisinin Research Center, Guangzhou University of Chinese Medicine, Guangzhou, China., Xia X; School of Pharmaceutical Sciences, Artemisinin Research Center, Guangzhou University of Chinese Medicine, Guangzhou, China., Liu F; The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Clinical Research Academy of Chinese Medicine, Guangzhou, China., Cai C; Chemical Engineering College, Nanjing University of Science & Technology, Nanjing, China., Deng T; School of Pharmaceutical Sciences, Artemisinin Research Center, Guangzhou University of Chinese Medicine, Guangzhou, China.; School of Medicine, Foshan University, Foshan, China.
Jazyk: angličtina
Zdroj: Luminescence : the journal of biological and chemical luminescence [Luminescence] 2024 Dec; Vol. 39 (12), pp. e70056.
DOI: 10.1002/bio.70056
Abstrakt: Trimethylamine oxide (TMAO), a microbial metabolite commonly found in foods, has been attracting increasing attention as it is associated with the risk of several diseases. Simple and accurate analytical methods are crucial for TMAO study. In the present study, we proposed a chemical reaction-based fluorescence assay for TMAO detection using synthetic small molecular probes. After systematic screening and optimization, the sensitive and selective quantification of TMAO has been achieved based on a fluorescence probe P6 (3-iodopropanyl group modified resorufin). Excellent linearity (R 2  = 0.997) was found between 6.25 and 50 μM, and the limit of detection (LOD) was 0.20 μM. Using this method, TMAO levels in several marine fishes and shellfishes have been successfully analyzed. The probe-based assay offers a simple and useful way for TMAO determination. The design is inspired by the unique oxidation reaction between TMAO and halogen, which opens a new perspective in the development of more advanced analytical assays for TMAO in the future.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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