Cotton cellulose nanofiber/chitosan scaffolds for skin tissue engineering and wound healing applications.

Autor:	Fayer L; Laboratory of Nanobiotechnology and Nanotoxicology, Department of Biology, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, Brazil., Vasconcellos R; Laboratory of Nanobiotechnology and Nanotoxicology, Department of Biology, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, Brazil., de Oliveira ER; Laboratory of Nanobiotechnology and Nanotoxicology, Department of Biology, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, Brazil., da Silva Almeida Ferreira C; Laboratory of Nanobiotechnology and Nanotoxicology, Department of Biology, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, Brazil., de Souza NLGD; Collegiate of Exact and Biotechnology Sciences, Federal University of Tocantis, Gurupi 77402-970, Brazil., Manhabosco TM; Department of Physics, Federal University of Ouro Preto, 35400-000 Ouro Preto, Brazil., de Oliveira LFC; Nucleus of Spectroscopy and Molecular Structure, Department of Chemistry, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, Brazil., Martins MA; National Laboratory of Nanotechnology for Agriculture, Embrapa Instrumentation, 13560-970 São Carlos, Brazil., Brandão HM; Laboratory of Applied Nanotechnology for Animal Production and Health, Brazilian Agricultural Research Corporation (EMBRAPA), 36038-330 Juiz de Fora, Brazil., Munk M; Laboratory of Nanobiotechnology and Nanotoxicology, Department of Biology, Federal University of Juiz de Fora, 36036-900 Juiz de Fora, Brazil.
Jazyk:	angličtina
Zdroj:	Biomedical materials (Bristol, England) [Biomed Mater] 2024 Dec 19; Vol. 20 (1). Date of Electronic Publication: 2024 Dec 19.
DOI:	10.1088/1748-605X/ad9da4
Abstrakt:	Chitosan (CS) is a promising polymeric biomaterial for use in scaffolds for in vitro skin models and wound dressings, owing to its non-antigenic and antimicrobial properties. However, CS often exhibits insufficient physicochemical properties, mechanical strength, and bioactivity, limiting its efficacy in demanding applications. To address these challenges, cotton cellulose nanofibers (CNFs) represent a promising nanomaterial for enhancing CS-based scaffolds in tissue engineering. CNF offers superior stiffness, and mechanical properties that enhance cellular adhesion and proliferation, both crucial for effective tissue regeneration and healing. This study aimed to develop and characterize a scaffold combining cotton CNF and CS, focusing on its cytocompatibility with human fibroblasts and keratinocytes. The cotton CNF/CS scaffold was fabricated using the casting technique, and its physicochemical properties and cellular compatibility were assessed in vitro . The results demonstrated that incorporating cotton CNF significantly enhanced the stability of the CS matrix. The CS scaffold with 1000 μg ml -1 of cotton CNF exhibited increased roughness and reduced rupture strain compared to the pure CS scaffold. The cotton CNF/CS scaffold effectively promoted the adhesion, viability, proliferation, migration, and collagen synthesis of skin cells. Notably, increased cell viability was observed in human fibroblasts cultured on scaffolds with higher concentrations of cotton CNF (100 and 1000 μg ml -1 ). Based on the findings, the cotton CNF/CS scaffold demonstrates enhanced physicochemical properties and bioactivity, making it a promising candidate for the development of in vitro human skin models and wound healing dressings. (© 2024 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.)
Databáze:	MEDLINE
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