Deep and dynamic metabolic and structural imaging in living tissues.

Autor: Liu K; Research Laboratory of Electronics, MIT, Cambridge, MA 02139, USA.; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA., Cao H; Research Laboratory of Electronics, MIT, Cambridge, MA 02139, USA.; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA., Shashaty K; Research Laboratory of Electronics, MIT, Cambridge, MA 02139, USA.; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA., Yu LY; Research Laboratory of Electronics, MIT, Cambridge, MA 02139, USA.; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA., Spitz S; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA., Pramotton FM; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA., Wan Z; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA., Kan EL; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA., Tevonian EN; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA., Levy M; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA., Lendaro E; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA., Kamm RD; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA.; Department of Mechanical Engineering, MIT, Cambridge, MA 02139, USA., Griffith LG; Department of Biological Engineering, MIT, Cambridge, MA 02139, USA.; Department of Mechanical Engineering, MIT, Cambridge, MA 02139, USA., Wang F; Department of Brain and Cognitive Sciences, MIT, Cambridge, MA 02139, USA., Qiu T; Research Laboratory of Electronics, MIT, Cambridge, MA 02139, USA.; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA., You S; Research Laboratory of Electronics, MIT, Cambridge, MA 02139, USA.; Department of Electrical Engineering and Computer Science, MIT, Cambridge, MA 02139, USA.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2024 Dec 13; Vol. 10 (50), pp. eadp2438. Date of Electronic Publication: 2024 Dec 11.
DOI: 10.1126/sciadv.adp2438
Abstrakt: Label-free imaging through two-photon autofluorescence of NAD(P)H allows for nondestructive, high-resolution visualization of cellular activities in living systems. However, its application to thick tissues has been restricted by its limited penetration depth within 300 μm, largely due to light scattering. Here, we demonstrate that the imaging depth for NAD(P)H can be extended to more than 700 μm in living engineered human multicellular microtissues by adopting multimode fiber-based, low repetition rate, high peak power, three-photon excitation of NAD(P)H at 1100 nm. This is achieved by having more than 0.5 megawatts peak power at the band of 1100 ± 25 nm through adaptively modulating multimodal nonlinear pulse propagation with a compact fiber shaper. Moreover, the eightfold increase in pulse energy enables faster imaging of monocyte behaviors in the living multicellular models. These results represent a substantial advance for deep and dynamic imaging of intact living biosystems. The modular design is anticipated to allow wide adoption for demanding imaging applications, including cancer research, immune responses, and tissue engineering.
Databáze: MEDLINE
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