Calcium signaling triggers early high humidity responses in Arabidopsis thaliana .

Autor: Hussain S; Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S3B2, Canada., Suda H; Department of Biochemistry and Molecular Biology, Saitama University, Saitama 338-8570, Japan., Nguyen CH; Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S3B2, Canada., Yan D; Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S3B2, Canada.; Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB T1J4B1, Canada., Toyota M; Department of Biochemistry and Molecular Biology, Saitama University, Saitama 338-8570, Japan.; Suntory Rising Stars Encouragement Program in Life Sciences, Suntory Foundation for Life Sciences, Kyoto 619-0284, Japan.; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China., Yoshioka K; Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S3B2, Canada.; The Centre for Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON M5S3B2, Canada., Nambara E; Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S3B2, Canada.; The Centre for Analysis of Genome Evolution and Function, University of Toronto, Toronto, ON M5S3B2, Canada.
Jazyk: angličtina
Zdroj: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Dec 17; Vol. 121 (51), pp. e2416270121. Date of Electronic Publication: 2024 Dec 11.
DOI: 10.1073/pnas.2416270121
Abstrakt: Plants need to adapt to fluctuating atmospheric humidity and respond to both high and low humidity. Despite our substantial understanding of plant responses to low humidity, molecular mechanisms underlying the high humidity (HH) response are much less well understood. In this study, we investigated early responses to HH in Arabidopsis . Expression of CYP707A3 , encoding an abscisic acid (ABA) 8'-hydroxylase, is induced by HH within 10 min, which leads to a decrease in foliar ABA level. We identified that the combined action of CAMTA3 and CAMTA2 transcription factors regulate this response. This regulation requires a calmodulin (CaM)-binding domain of CAMTA3. Transcriptomes of HH-regulated genes are enriched in those related to calcium signaling, including cyclic nucleotide-gated ion channels (CNGCs). Moreover, HH induces CNGC2- and CNGC4-mediated increases in cytosolic Ca 2+ concentrations in leaves within a few minutes. We also found that CNGC2, CNGC4, and CAMTAs participate in HH-induced hyponastic movement of petioles. Taken together, our results indicate that CNGC2/CNGC4-Ca 2+ -CaM-CAMTA3/CAMTA2 acts as a primary regulatory module to trigger downstream HH responses.
Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze: MEDLINE