A novel mechanism of microbial attachment: The flagellar pump of Giardia lamblia .

Autor:	Picou TJ; Department of Biology, Georgetown University, Washington, DC, USA., Luo H; Department of Biology, Georgetown University, Washington, DC, USA., Polackwich RJ; Department of Physics and Institute for Soft Matter Synthesis and Metrology, Georgetown University, Washington, DC, USA., Gabilondo BB; Department of Physics, The Ohio State University, Columbus, OH, USA., McAllister RG; Department of Physics and Institute for Soft Matter Synthesis and Metrology, Georgetown University, Washington, DC, USA., Gagnon DA; Department of Physics and Institute for Soft Matter Synthesis and Metrology, Georgetown University, Washington, DC, USA., Powers TR; School of Engineering and Department of Physics, Brown University, Providence, RI, USA., Elmendorf HG; Department of Biology, Georgetown University, Washington, DC, USA., Urbach JS; Department of Physics and Institute for Soft Matter Synthesis and Metrology, Georgetown University, Washington, DC, USA.
Jazyk:	angličtina
Zdroj:	PNAS nexus [PNAS Nexus] 2024 Nov 29; Vol. 3 (12), pp. pgae545. Date of Electronic Publication: 2024 Nov 29 (Print Publication: 2024).
DOI:	10.1093/pnasnexus/pgae545
Abstrakt:	The ability of microbes to attach to biological and inert substrates is a necessary prerequisite for colonization of new habitats. In contrast to well-characterized mechanisms that rely on specific or nonspecific chemical interactions between microbe and substrate, we describe here an effective hydrodynamic mechanism of attachment that relies on fluid flow generated by the microbe. The microbe Giardia lamblia , a flagellated protozoan parasite, naturally attaches to the microvilliated surface of the small intestine but is also capable of attaching indiscriminately to a wide range of natural and artificial substrates. By tracking fluorescent quantum dots, we demonstrate a persistent flow between the parasite and substrate generated by a pair of Giardia flagella. Using both experimental measures and computational modeling, we show that the negative pressure generated by this fluid flow is sufficient to generate the previously measured force of attachment. We further show that this dynamically generated negative pressure allows Giardia to attach to both solid and porous surfaces, thereby meeting the real-world demands of attachment to the microvilliated surface of intestinal cells. These findings provide experimental support for a hydrodynamic model of attachment that may be shared by other ciliated and flagellated microbes. (© The Author(s) 2024. Published by Oxford University Press on behalf of National Academy of Sciences.)
Databáze:	MEDLINE
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