ASAP1 promotes extrahepatic cholangiocarcinoma progression by regulating the Wnt/β-catenin pathway in vitro and in vivo.

Autor: He J; Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University Shanghai 200032, China.; Department of General Surgery, Huadong Hospital Affiliated to Fudan University Shanghai 200040, China., Liu H; Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University Shanghai 200032, China., Cai J; Department of General Surgery, Huadong Hospital Affiliated to Fudan University Shanghai 200040, China., Shen S; Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University Shanghai 200032, China., Wang J; Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University Shanghai 200032, China., Liu H; Department of General Surgery, Zhongshan Hospital Affiliated to Fudan University Shanghai 200032, China.
Jazyk: angličtina
Zdroj: American journal of cancer research [Am J Cancer Res] 2024 Nov 15; Vol. 14 (11), pp. 5178-5192. Date of Electronic Publication: 2024 Nov 15 (Print Publication: 2024).
DOI: 10.62347/RKQX3504
Abstrakt: This study sought to identify the relationship between ADP-ribosylation factor GTPase-activating protein (ASAP1) expression and clinical outcomes in extrahepatic cholangiocarcinoma (EHCC) patients. Quantitative real-time PCR (qRT-PCR), Western blotting, and immunohistochemistry were used to analyze the expression of ASAP1 in cholangiocarcinoma (CC) tissue samples and cell lines. The survival rate and clinicopathological characteristics of CC patients were also examined. Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EdU) assays were used to detect cell proliferation. Flow cytometry was used to assess the cell cycle distribution. Both in vitro and in vivo experiments showed that ASAP1 knockdown decreased cell proliferation, inhibited cell cycle progression, and increased apoptosis. ASAP1 regulates Wnt/β-catenin pathway activity in CC, promoting cell migration, and invasion in culture; and promotes tumor development in vivo . ASAP1 plays a key role in EHCC tumor development and could serve as a potential therapeutic target for EHCC.
Competing Interests: None.
(AJCR Copyright © 2024.)
Databáze: MEDLINE
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