Stepwise molecular specification of excitatory synapse diversity onto cerebellar Purkinje cells.

Autor: Paul MA; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, Paris, France., Sigoillot SM; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, Paris, France., Marti L; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, Paris, France., Urra Quiroz FJ; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, Paris, France.; Inovarion, Paris, France., Delagrange M; Plateforme qPCR-HD-GPC, Institut de Biologie de l'Ecole Normale Supérieure, Paris, France., Cheung HW; Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA., Martinelli DC; Department of Neuroscience, University of Connecticut Health, Farmington, CT, USA.; The Connecticut Institute for the Brain and Cognitive Sciences (IBACS), Storrs, CT, USA., Oriol E; Laboratoire de Physique de l'Ecole Normale Supérieure, CNRS, Ecole Normale Supérieure, PSL University, Sorbonne Université, Université de Paris, Paris, France., Hakim V; Laboratoire de Physique de l'Ecole Normale Supérieure, CNRS, Ecole Normale Supérieure, PSL University, Sorbonne Université, Université de Paris, Paris, France., Mailly P; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, Paris, France., Selimi F; Center for Interdisciplinary Research in Biology (CIRB), Collège de France, Université PSL, CNRS, INSERM, Paris, France. fekrije.selimi@college-de-france.fr.
Jazyk: angličtina
Zdroj: Nature neuroscience [Nat Neurosci] 2024 Dec 10. Date of Electronic Publication: 2024 Dec 10.
DOI: 10.1038/s41593-024-01826-w
Abstrakt: Brain function relies on the generation of a large variety of morphologically and functionally diverse, but specific, neuronal synapses. Here we show that, in mice, the initial formation of synapses on cerebellar Purkinje cells involves a presynaptic protein-CBLN1, a member of the C1q protein family-that is secreted by all types of excitatory inputs. The molecular program then evolves only in one of the Purkinje cell inputs, the inferior olivary neurons, with the additional expression of the presynaptic secreted proteins C1QL1, CRTAC1 and LGI2. These molecules work in concert to specify the mature connectivity pattern on the Purkinje cell target. These results show that some inputs actively and gradually specify their synaptic molecular identity, while others rely on the 'original molecular code'. Thus, the molecular specification of excitatory synapses, crucial for proper circuit function, is acquired in a stepwise manner during mouse postnatal development and obeys input-specific rules.
Competing Interests: Competing interests: The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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