CD55 characterizes regulatory T cells with reduced functionality and is downregulated in rheumatoid arthritis.

Autor: Bahabayi A; Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China., Xiong Z; Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China., Li Q; Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China., Wang G; Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China., Liu R; Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China., Zhang K; Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China., Zhang Z; Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China., Gao Y; Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China., Wang P; Department of Immunology, NHC Key Laboratory of Medical Immunology (Peking University), Medicine Innovation Center for Fundamental Research on Major Immunology-related Diseases, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China; Peking University Center for Human Disease Genomics, Peking University Health Science Center, Beijing, China., Liu C; Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China. Electronic address: liuchen-best@pku.edu.cn.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2025 Jan 03; Vol. 145, pp. 113822. Date of Electronic Publication: 2024 Dec 10.
DOI: 10.1016/j.intimp.2024.113822
Abstrakt: Objective: This study aimed to investigate the role of CD55 in regulatory T cells (Tregs) and clarify its clinical relevance in rheumatoid arthritis (RA).
Methods: Flow cytometry was used to examine the expression of Helios and CTLA-4 in CD55 + and CD55- Tregs in mouse peripheral blood and spleen. FoxP3 EGFP mice were employed to analyze the in vitro inhibitory function of CD55 + and CD55-Tregs. We compared CD55 expression and function in control and CIA mice. Additionally, the expression of Helios, PD-1, and TIGIT in CD55 + Tregs was examined in healthy controls and RA patients. Correlation analysis and receiver operating characteristic (ROC) curves were used to assess the clinical utility of CD55-related T cell subgroups in RA diagnosis.
Results: High CD55 expression was observed in CD4 + T cells and Tregs, with significantly higher levels in peripheral blood than in the spleen in mice. CD55-Tregs exhibited increased expression of Helios and CTLA-4, and a more pronounced suppressive function compared to CD55 + Tregs in mice. Additionally, CD55 expression in Tregs from peripheral blood and spleen of CIA mice was significantly reduced. In humans, peripheral blood CD55- Tregs expressed higher levels of Helios and TIGIT. Moreover, CD55 + Tregs were markedly reduced in RA patients and correlated with clinical indicators of RA, demonstrating potential as diagnostic markers.
Conclusions: CD55 + Tregs represent a subset of Tregs with weaker functionality and were decreased in RA and could assist the diagnosis of RA.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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