A glutamine synthetase-Dof transcription factor module regulates nitrogen remobilization from source to sink tissues in tea plants.

Autor:	Liu MY; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.; Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China., Jiao Z; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.; Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China., Lou H; State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, Zhejiang 311300, China., Tang D; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.; College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China., Tian X; Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China., Zhou BW; Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China., Ruan J; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.; Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China., Fernie AR; Max-Planck-Institute of Molecular Plant Physiology, Am Muehlenberg 1, 14476 Potsdam-Golm, Germany., Zhang Q; Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China.; Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Hangzhou 310008, China.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2024 Dec 23; Vol. 197 (1).
DOI:	10.1093/plphys/kiae644
Abstrakt:	Nitrogen (N) remobilization from mature leaves to new shoots (NSs) is closely related to the quality of green tea in the spring season, which subsequently determines its economic value. However, the underlying N remobilization mechanism remains poorly understood. Here, we demonstrate that >80% of the recovered 15N was partitioned in the first mature leaves that supply NSs. N-remobilization efficiency (NRE) from mature leaves to NSs varied significantly among tea cultivars. N-fertilization level and NRE showed a significantly positive correlation. Based on weighted gene co-expression network analysis, glutamate metabolism-related genes, including glutamine synthetase genes, CsGSs, were dissected from the interaction network of hub genes regulating N remobilization. Gene expression patterns and the localization of CsGS1.1 in the cytosol and vascular tissue suggest its potential role in N remobilization. Consistent with these findings, source-to-sink N remobilization at the reproductive stage was enhanced in transgenic CsGS1.1-overexpressing plants. Furthermore, we demonstrated that the Dof transcription factor CsDof16 directly binds to the -526 to -426 region of the CsGS1.1 promoter, thereby activating its transcription and regulating N remobilization. Taken together, our findings suggest that the CsDof16-CsGS1.1 module regulates the remobilization of N in the form of glutamate/glutamine from mature leaves to NSs, constituting an important control point in the regulation of source-to-sink N partitioning in tea plants. Our findings can be employed to reduce fertilizer application and promote the development of sustainable tea production. Competing Interests: Conflict of interest statement. None declared. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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