Protocol for quantifying xenografted human cancer cells in zebrafish larvae using Cellpose.

Autor:	Köpke K; European Research Institute for the Biology of Ageing, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands. Electronic address: k.kopke@umcg.nl., Lembke CS; European Research Institute for the Biology of Ageing, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands., Oosterhof N; European Research Institute for the Biology of Ageing, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands., Dijkstra ESC; European Research Institute for the Biology of Ageing, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands., Paridaen JTML; European Research Institute for the Biology of Ageing, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2024 Dec 20; Vol. 5 (4), pp. 103479. Date of Electronic Publication: 2024 Dec 09.
DOI:	10.1016/j.xpro.2024.103479
Abstrakt:	Here, we present a protocol for quantifying xenografted human glioblastoma stem cells (GSCs) in zebrafish larvae. We first describe steps for orthotopic xenotransplantation of GSCs into the midbrain of zebrafish larvae, immunofluorescent labeling, and confocal imaging. We then detail procedures for GSC quantification using the Cellpose algorithm. This protocol provides a technique for the semi-automatic segmentation and quantification of human cancer cells in xenograft experiments. With this approach, cancer cell survival and proliferation can be determined in an unbiased manner. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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