Photoionization of pyrenemethylamine-labeled oligosaccharides: a new MALDI-TOF precursor ion-type for efficient fragmentation.

Autor: Franz AH; Department of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA. afranz@pacific.edu., Ta N; Department of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA., Nguyen E; Department of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA., Bromley K; Department of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA., Rosenblatt H; Department of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA., Fabro A; Department of Chemistry, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA., Lin-Cereghino G; Department of Biology, University of the Pacific, 3601 Pacific Avenue, Stockton, CA, 95211, USA.
Jazyk: angličtina
Zdroj: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2024 Dec 10. Date of Electronic Publication: 2024 Dec 10.
DOI: 10.1007/s44211-024-00700-w
Abstrakt: Oligosaccharides were covalently labeled with 1-pyrenemethylamine (1-PMA) in 15 min at 75 °C with high yield, separated by quantitative HPLC in less than 20 min, and characterized by off-line Matrix-Assisted Laser Desorption/Ionization (MALDI) Time-of-Flight (TOF) mass spectrometry (MS). A new MALDI mass spectral precursor ion for fragmentation studies was observed from 2,5-dihydroxybenzoic acid matrix (DHB, hydroquinone/benzoquinone redox system) through photo-induced reductive elimination. The resulting high-energy protonated glycosylamine provided excellent fragmentation efficiency (Y-, B-ions, and combination losses), with ions covering almost the entire structure of several oligosaccharides at the low picomol level. With the new method, glycans from commercially available standard glycoproteins and glycans from bioengineered β-lactoglobulin expressed in the bgs13 mutant of Pichia pastoris were analyzed.
Competing Interests: Declarations. Conflict of interest: The authors declare that they have no known competing financial interests or personal relationships that could have adversely influenced the work reported in this paper.
(© 2024. The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry.)
Databáze: MEDLINE
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