Engineered bacteria that self-assemble bioglass polysilicate coatings display enhanced light focusing.

Autor:	Sidor LM; Department of Biology, University of Rochester, Rochester, NY 14627., Beaulieu MM; Department of Physics and Astronomy, University of Rochester, Rochester, NY 14627., Rasskazov I; Institute of Optics, University of Rochester, Rochester, NY 14627., Acarturk BC; Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, Boulder, CO 80309., Ren J; Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, Boulder, CO 80309., Jenen E; Department of Biology, University of Rochester, Rochester, NY 14627., Kamoen L; Department of Biotechnology, Delft University of Technology, Delft 2629 HZ, The Netherlands., Vitali MV; Department of Biotechnology, Delft University of Technology, Delft 2629 HZ, The Netherlands., Carney PS; Institute of Optics, University of Rochester, Rochester, NY 14627., Schmidt GR; Institute of Optics, University of Rochester, Rochester, NY 14627., Srubar WV 3rd; Department of Civil, Environmental, and Architectural Engineering, University of Colorado Boulder, Boulder, CO 80309.; Materials Science and Engineering Program, University of Colorado Boulder, Boulder, CO 80309., Abbondanzieri EA; Department of Biology, University of Rochester, Rochester, NY 14627., Meyer AS; Department of Biology, University of Rochester, Rochester, NY 14627.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Dec 17; Vol. 121 (51), pp. e2409335121. Date of Electronic Publication: 2024 Dec 10.
DOI:	10.1073/pnas.2409335121
Abstrakt:	Cutting-edge photonic devices frequently rely on microparticle components to focus and manipulate light. Conventional methods used to produce these microparticle components frequently offer limited control of their structural properties or require low-throughput nanofabrication of more complex structures. Here, we employ a synthetic biology approach to produce environmentally friendly, living microlenses with tunable structural properties. We engineered Escherichia coli bacteria to display the silica biomineralization enzyme silicatein from aquatic sea sponges. Our silicatein-expressing bacteria can self-assemble a shell of polysilicate "bioglass" around themselves. Remarkably, the polysilicate-encapsulated bacteria can focus light into intense nanojets that are nearly an order of magnitude brighter than unmodified bacteria. Polysilicate-encapsulated bacteria are metabolically active for up to 4 mo, potentially allowing them to sense and respond to stimuli over time. Our data demonstrate that synthetic biology offers a pathway for producing inexpensive and durable photonic components that exhibit unique optical properties. Competing Interests: Competing interests statement:L.M.S. and A.S.M. Modified bacteria and methods of use for bioglass microlenses. Provisional US patent application No. 63/338,490, filed May 5, 2022. International Patent Cooperation Treaty patent application filed May 5, 2023.
Databáze:	MEDLINE
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