| Autor: |
Lee SR; Department of Chemistry, Princeton University, Princeton, New Jersey 08544, United States.; College of Pharmacy and Research Institute for Drug Development, Pusan National University, Busan 46241, Republic of Korea., Gallant É; Department of Chemistry, Princeton University, Princeton, New Jersey 08544, United States., Seyedsayamdost MR; Department of Chemistry, Princeton University, Princeton, New Jersey 08544, United States.; Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, United States. |
| Abstrakt: |
It is now well-established that microbial genomes carry sparingly expressed biosynthetic gene clusters (BGCs) that need to be induced in order to characterize their products. To do so, we herein subjected two well-known producers, Burkholderia plantarii and Burkholderia gladioli , to high-throughput elicitor screening (HiTES), an emerging approach for accessing the products of these "cryptic" BGCs. Both organisms have previously been examined extensively in liquid cultures. We therefore applied HiTES on agar and found several novel natural products that are only produced in this format and not in liquid cultures. Most notably we found two metabolites, termed burkethyl A and B, that contain an unusual m -ethylbenzoyl group and for which we identified the cognate BGC using bioinformatic and genetic studies. Our results indicate that agar-based HiTES is a promising approach for natural product discovery and are in line with the notion that even "drained" strains remain sources of new metabolites as long as alternative approaches are employed. |
