The Chick Embryo Chorioallantoic Membrane (CAM) Assay: A Novel Experimental Model in Dental Research.
| Autor: | Caplar BD; Department of Prostheses Technology and Dental Materials, Faculty of Dental Medicine, Dental Research Center Using Conventional and Alternative Technologies, 'Victor Babes' University of Medicine and Pharmacy, Timisoara, ROU., Togoe MM; Department of Prostheses Technology and Dental Materials, Faculty of Dental Medicine, Dental Research Center Using Conventional and Alternative Technologies, 'Victor Babes' University of Medicine and Pharmacy, Timisoara, ROU., Ribatti D; Department of Translational Biomedicine and Neuroscience, University of Bari Medical School, Bari, ITA., Pop D; Department of Prostheses Technology and Dental Materials, Faculty of Dental Medicine, Dental Research Center Using Conventional and Alternative Technologies, 'Victor Babes' University of Medicine and Pharmacy, Timisoara, ROU., Sinescu C; Department of Dentistry, Faculty of Dentistry, 'Victor Babes' University of Medicine and Pharmacy, Timisoara, ROU., Rominu M; Department of Prostheses Technology and Dental Materials, Faculty of Dental Medicine, Dental Research Center Using Conventional and Alternative Technologies, 'Victor Babes' University of Medicine and Pharmacy, Timisoara, ROU., Petrescu EL; Department of Prostheses Technology and Dental Materials, Faculty of Dental Medicine, Dental Research Center Using Conventional and Alternative Technologies, 'Victor Babes' University of Medicine and Pharmacy, Timisoara, ROU., Negrutiu ML; Department of Prostheses Technology and Dental Materials, Faculty of Dental Medicine, Dental Research Center Using Conventional and Alternative Technologies, 'Victor Babes' University of Medicine and Pharmacy, Timisoara, ROU., Melnic E; Department of Pathology, Nicolae Testemitanu State University of Medicine and Pharmacy, Chișinău, MDA., Cimpean AM; Department of Microscopic Morphology/Histology, 'Victor Babes' University of Medicine and Pharmacy, Timisoara, ROU. |
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| Jazyk: | angličtina |
| Zdroj: | Cureus [Cureus] 2024 Nov 29; Vol. 16 (11), pp. e74714. Date of Electronic Publication: 2024 Nov 29 (Print Publication: 2024). |
| DOI: | 10.7759/cureus.74714 |
| Abstrakt: | Animal experimental models are ruled out by respecting the 3Rs (Replacement, Reduction, Refinement) rules which governed the experimental research for decades with an increased tendency to minimize as much as it is possible any pain suffering or distress that the animals might feel. The chick embryo chorioallantoic membrane (CAM) model is an alternative to other experimental models due to its superior properties compared to other animal models. The CAM is painless by itself due to the lack of innervation and has no immune cells till the 11th day of incubation. Thus, it is extensively used for implanting malignant tumors and assessing them in relation to their metastatic and angiogenic potential. Also, various biomaterials from collagen to hard scaffolds can be implanted on the CAM surface and analyzed mainly related to their property of inducing inflammation. Dental research often uses mouse or rabbit models for experimental purposes. Different surgical techniques from experimentally induced periodontal disease to experimental dental implants may cause pain and suffering to animals. Due to all these arguments, the CAM model is a quick, cheap, and reliable alternative to other animal experimental models used in dental research. Despite its usefulness as an experimental model for different applications, ranging from inflammation studies to cancer research, the CAM model is insufficiently used in dental research. Currently, about 135 studies pertaining to this issue are available in PubMed, the majority of which focus on the reactivity of CAM vessels to various materials employed in dentistry. Limited data exist about the capacity of the CAM to promote osteogenic differentiation of dental stem cells or to enhance biomaterial integration into novel tissue architectures. The present review critically analyzed the use of the CAM model as an experimental tool in dental research. We selected from PubMed all the papers having as topic the CAM in dentistry by searching based on the following keywords: " chorioallantoic membrane, dentistry" or "chorioallantoic membrane, dental ". We focused on discussing the benefits and limitations of the CAM model in dental studies and its prospective role as a preclinical instrument for the assessment of dental tissues, biomaterials, or different dentistry-related substances prior to their use for various purposes in dental clinical practice. The impact of the CAM model-derived preclinical findings on clinical practice will be also stated by mentioning "pros and cons" arguments. The last part of the present paper reviewed the perspective of CAM assay used in combination with other experimental techniques such as tooth organoids and also the strengths and weaknesses of other species CAM assays recently developed in ostrich and Nile crocodile CAMs. Competing Interests: Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. (Copyright © 2024, Caplar et al.) |
| Databáze: | MEDLINE |
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