Propionimicrobium lymphophilum in urine of children with monosymptomatic nocturnal enuresis.

Autor:	Nishizaki N; Department of Pediatrics, Juntendo University Urayasu Hospital, Urayasu, Chiba, Japan., Oshiro S; AMR Research Laboratory, Juntendo Advanced Research Institute for Health Science, Juntendo University School of Medicine, Tokyo, Japan., Tohya M; Division of Food Safety Information, National Institute of Health Sciences, Kawasaki, Kanagawa, Japan., Watanabe S; Department of Microbiome Research, Juntendo University Graduate School of Medicine, Tokyo, Japan., Okazaki T; Department of Pediatric Surgery, Juntendo University Urayasu Hospital, Urayasu, Chiba, Japan., Takahashi K; Department of Pediatrics, Juntendo University Urayasu Hospital, Urayasu, Chiba, Japan., Kirikae T; AMR Research Laboratory, Juntendo Advanced Research Institute for Health Science, Juntendo University School of Medicine, Tokyo, Japan.; Department of Microbiome Research, Juntendo University Graduate School of Medicine, Tokyo, Japan., Shimizu T; Department of Pediatrics and Adolescent Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 Nov 25; Vol. 14, pp. 1377992. Date of Electronic Publication: 2024 Nov 25 (Print Publication: 2024).
DOI:	10.3389/fcimb.2024.1377992
Abstrakt:	Background: Despite a unique microbiome in urine, the relationship between nocturnal enuresis and the urobiome remains unclear. This study aimed to compare the presence of specific bacterial species in the urine of children with and without nocturnal enuresis. Methods: We used 16S ribosomal RNA gene sequencing to analyze the urobiome in urine samples obtained from the two groups of children. The presence of Propionimicrobium lymphophilum was examined using real-time PCR in the urine of 25 children diagnosed with monosymptomatic nocturnal enuresis (MNE), and 17 children without this condition. Results: Children with MNE exhibited a significantly higher prevalence of P. lymphophilum : 16 out of 25 (64.0%) compared to 4 out of 17 (23.5%) in the control group. Among children with frequent bedwetting, there was a significantly higher prevalence of P. lymphophilum ;15 out of 16 (93.8%) compared to 2 out of 9 (22.2%) in those with infrequent bedwetting. Bacterial culture tests confirmed the anaerobic growth of P. lymphophilum isolates from urine samples of two PCR-positive patients with MNE. These isolates were found to be susceptible to ampicillin. Conclusion: These findings suggest that P. lymphophilum may be associated with chronic urinary tract infections and potentially contribute to the development of MNE in children. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Nishizaki, Oshiro, Tohya, Watanabe, Okazaki, Takahashi, Kirikae and Shimizu.)
Databáze:	MEDLINE
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