Tannins and copper sulphate as antimicrobial agents to prevent contamination of Posidonia oceanica seedling culture for restoration purposes.

Autor:	Alagna A; Department of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Palermo, Italy.; National Biodiversity Future Centre (NBFC), Palermo, Italy., Giacalone VM; National Biodiversity Future Centre (NBFC), Palermo, Italy.; Institute for the Anthropic impacts and Sustainability in Marine Environment, IAS-CNR, Capo Granitola, Italy., Zenone A; Department of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Palermo, Italy.; National Biodiversity Future Centre (NBFC), Palermo, Italy.; Institute for the Anthropic Impacts and Sustainability in Marine Environment, IAS-CNR, Palermo, Italy., Martinez M; Institute for the Anthropic Impacts and Sustainability in Marine Environment, IAS-CNR, Palermo, Italy., D'Anna G; National Biodiversity Future Centre (NBFC), Palermo, Italy.; Institute for the Anthropic Impacts and Sustainability in Marine Environment, IAS-CNR, Castellammare del Golfo, Italy., Buffa G; National Biodiversity Future Centre (NBFC), Palermo, Italy.; Institute for the Anthropic impacts and Sustainability in Marine Environment, IAS-CNR, Capo Granitola, Italy., Cavalca CJ; Institute for the Anthropic impacts and Sustainability in Marine Environment, IAS-CNR, Capo Granitola, Italy., Poli A; National Biodiversity Future Centre (NBFC), Palermo, Italy.; Department of Life Sciences and Systems Biology, University of Torino, Mycotheca Universitatis Taurinensis (MUT), Torino, Italy., Varese GC; National Biodiversity Future Centre (NBFC), Palermo, Italy.; Department of Life Sciences and Systems Biology, University of Torino, Mycotheca Universitatis Taurinensis (MUT), Torino, Italy., Prigione VP; National Biodiversity Future Centre (NBFC), Palermo, Italy.; Department of Life Sciences and Systems Biology, University of Torino, Mycotheca Universitatis Taurinensis (MUT), Torino, Italy., Badalamenti F; National Biodiversity Future Centre (NBFC), Palermo, Italy.; Institute for the Anthropic Impacts and Sustainability in Marine Environment, IAS-CNR, Palermo, Italy.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2024 Nov 25; Vol. 15, pp. 1433358. Date of Electronic Publication: 2024 Nov 25 (Print Publication: 2024).
DOI:	10.3389/fpls.2024.1433358
Abstrakt:	Seed-based restoration methods are increasingly recognized as a relevant tool contributing to halt and reverse the loss of seagrass meadows while providing genetic and evolutionary benefit for the conservation of these habitats. Ad-hoc protocols aimed at maximizing the survival of plantlets obtained from seeds in cultivation systems are therefore required. Previous trials of seedling culture of Posidonia oceanica , the dominant seagrass of the Mediterranean Sea, recorded up to 40% loss due to mould development. In this study we aim to (i) identify the putative causal agents of seed decay and (ii) test the efficacy of copper sulphate (0.2 and 2 ppm) and of tannin-based products derived from chestnut, tara and quebracho in reducing seed and seedling decay, while assessing possible phytotoxic effects on plant development. Halophytophthora lusitanica, H. thermoambigua and a putative new Halophytophtora species were identified as possible causal agents of seed loss. The antimicrobial agents (copper and tannins) reduced seed contamination by 20%, although copper sulphate at 2 ppm strongly inhibited the root growth. Among tannins, chestnut and tara reduced seeds germination by up to 75% and decreased shoot and root development, while quebracho showed a less severe phytotoxic effect. The use of copper sulphate at 0.2 ppm is therefore recommended to prevent P. oceanica seedling loss in culture facilities since it reduces seed contamination with no phytotoxic effects. Our results contribute to improving the seedling culture of one the key species of the Mediterranean Sea, increasing propagule availability for restoration purposes. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Alagna, Giacalone, Zenone, Martinez, D’Anna, Buffa, Cavalca, Poli, Varese, Prigione and Badalamenti.)
Databáze:	MEDLINE
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