Genetic characterization and clonal analysis of carbapenemase-producing Escherichia coli and Klebsiella pneumoniae from canine and human origins.

Autor:	Khine NO; Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Shah AA; Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Chatsuwan T; Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand., Yindee J; Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Supimon N; Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Saenkankam I; Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand., Hampson DJ; School of Veterinary Medicine, Murdoch University, Perth, WA, Australia., Prapasarakul N; Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.; Center of Excellency in Diagnosis and Monitoring of Animal Pathogens (CEDMAP), Bangkok, Thailand.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2024 Nov 25; Vol. 11, pp. 1464934. Date of Electronic Publication: 2024 Nov 25 (Print Publication: 2024).
DOI:	10.3389/fvets.2024.1464934
Abstrakt:	Introduction: Carbapenem-resistant Enterobacterales (CRE), particularly carbapenemase-producing Escherichia coli and Klebsiella pneumoniae , pose a significant global health challenge due to their resistance to last-resort antibiotics. This study investigates the genetic characteristics and clonal relationships of CRE isolated from canine and human clinical samples in Bangkok to understand potential interspecies transmission. Methods: Fifty-two CRE isolates were collected from 477 clinical samples from dogs and humans at Chulalongkorn University between 2017-2021. Bacterial species were identified using MALDI-TOF, and antimicrobial resistance was confirmed through broth microdilution testing. Genetic analyses included plasmid replicon typing, multilocus sequence typing (MLST), whole genome sequencing (WGS), and pulsed-field gel electrophoresis (PFGE) to assess resistance genes and clonal relatedness. Results: CRE isolates from both species exhibited genetic variability with high ARG counts, particularly in human isolates. MLST identified ST410 in most E. coli isolates from both dogs and humans, and IncFIA/IncFIB plasmids were predominant among blaNDM-positive isolates. PFGE patterns and SNP analysis showed no clonal relationship between canine and human isolates, suggesting independent acquisition pathways for CRE in the two hosts. Discussion: The study highlights the absence of direct clonal transmission between canine and human isolates but reveals overlapping sequence types and plasmid types. The findings underscore the potential for interspecies transmission under certain conditions, emphasizing the importance of a One Health approach for monitoring CRE in both human and animal populations. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Khine, Shah, Chatsuwan, Yindee, Supimon, Saenkankam, Hampson and Prapasarakul.)
Databáze:	MEDLINE
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