Validation of plasmonic-based biosensors for rapid and in depth characterization of monoclonal antibodies directed against rabbit haemorrhagic and foot-and-mouth disease viruses in biological samples.

Autor: Urbinati C; Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy., Pezzoni G; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna and WOAH, Reference Laboratory for Rabbit Haemorrhagic Disease, Brescia, Italy., Cavadini P; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna and WOAH, Reference Laboratory for Rabbit Haemorrhagic Disease, Brescia, Italy., Giovanni VD; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna and WOAH, Reference Laboratory for Rabbit Haemorrhagic Disease, Brescia, Italy., Capucci L; Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna and WOAH, Reference Laboratory for Rabbit Haemorrhagic Disease, Brescia, Italy., Rusnati M; Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy; Consorzio Interuniversitario Biotecnologie (CIB), Unit of Brescia, Brescia, Italy. Electronic address: marco.rusnati@unibs.it.
Jazyk: angličtina
Zdroj: Methods (San Diego, Calif.) [Methods] 2024 Dec 09; Vol. 234, pp. 85-92. Date of Electronic Publication: 2024 Dec 09.
DOI: 10.1016/j.ymeth.2024.12.003
Abstrakt: ELISA and RT-PCR represent the standard tools for the sensitive identification of viruses in biological samples, but they lack the capacity to finely characterize the binding of viruses or viral antigens to monoclonal antibodies (MAbs). Biosensing technologies are gaining increasing importance as powerful MAb characterization tools in the field of virology. Surface plasmon resonance (SPR) is an optical biosensing technology already used for the in depth characterization of MAbs of diagnostic and therapeutic value. Rabbit haemorrhagic disease virus (RHDV) and foot-and-mouth disease virus (FMDV) are top veterinary issues for which the development of novel methods aimed at the characterization of antiviral MAbs represents a priority with important livestock healthcare and economic implications. With these premises in mind, here we prepared a series of SPR biosensors by immobilizing RHDV2 or its 6S subunit by different strategies that were then used to characterize the binding capacity of a panel of anti-RHDV2 MAbs. From the comparison of the results obtained, the biosensor composed of intact RHDV2 captured with catcher-MAb covalently immobilized to the surface showed the best analytical performances. To evaluate the versatility of the biosensor, the same strategy was then adopted using FMVD in cell extracts. The results obtained are discussed in view of the exploitation of SPR in the rapid and resilient fine characterization of antiviral MAbs for diagnostic or therapeutic purposes in the field of animal virology.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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