| Autor: |
Quirié A; INSERM UMR1093-CAPS, Université de Bourgogne, UFR Sciences de Santé, F-21000 Dijon, France., Mor D; Université de Franche-Comté, UMR INSERM 1322 LINC, F-25000 Besançon, France., Méloux A; INSERM UMR1093-CAPS, Université de Bourgogne, UFR Sciences de Santé, F-21000 Dijon, France., Etievant A; Université de Franche-Comté, UMR INSERM 1322 LINC, F-25000 Besançon, France., Garnier P; INSERM UMR1093-CAPS, Université de Bourgogne, UFR Sciences de Santé, F-21000 Dijon, France., Totoson P; Université de Franche-Comté, EFS, INSERM, UMR 1098 RIGHT, F-25000 Besançon, France., Wirtz J; INSERM UMR1093-CAPS, Université de Bourgogne, UFR Sciences de Santé, F-21000 Dijon, France., Prigent-Tessier A; INSERM UMR1093-CAPS, Université de Bourgogne, UFR Sciences de Santé, F-21000 Dijon, France., Marie C; INSERM UMR1093-CAPS, Université de Bourgogne, UFR Sciences de Santé, F-21000 Dijon, France., Demougeot C; Université de Franche-Comté, EFS, INSERM, UMR 1098 RIGHT, F-25000 Besançon, France. |
| Abstrakt: |
The present study investigated the role of endothelial BDNF in cognition. Male adult mice with a selective knockout of BDNF in endothelial cells ( BDNF ECKO ) and their wild-type littermates (WT) were subjected to tests for detection of anxiety- and depression-like behaviors and impaired recognition memory. Neuronal activity and synaptogenesis were assessed from hippocampal levels of c-fos and synaptophysin, respectively, and cerebral capillary density from forebrain levels of CD31. BDNF/TrkB (tropomyosin-related kinase type B) receptor signaling was investigated through hippocampal levels of BDNF and activated TrkB receptors coupled with their immunolabelling by neurons and endothelial cells from both cerebrovascular fractions enriched in capillaries and hippocampal arterioles. Endothelial nitric oxide (NO) production was assessed from expression of endothelial NO synthase phosphorylated at serine 1177. BDNF ECKO mice exhibited anxio-depressive phenotype, impaired memory and reduced synaptogenesis. Neither neuronal activity, neuronal BDNF/TrkB signaling nor capillary density differed between BDNF ECKO and WT mice. However, endothelial-activated TrkB receptors as well as endothelial NO production and hippocampal BDNF levels were lower in BDNF ECKO than WT mice. We conclude that endothelial BDNF is involved in cognition through mechanisms independent of neuronal BDNF/TrkB signaling and that endothelial NO might be a driver of the pro-cognitive effect of endothelial BDNF. |
